Equipments Details
Description
The MHFI Olympus FV1000 laser scanning confocal microscopy system is capable of obtaining multicolor, 3D images of living cells in a controlled environment. It can also perform spectral unmixing to allow resolution of fluorescence overlap and Total Internal Reflection Fluorescence (TIRF) microscopy. Laser lines available include 405, 458, 488, 515, 559 and 635nm.
Confocal laser scanning microscopy permits high resolution optical sectioning without compromising specimen integrity. Some advantages of using confocal microscopy include:
- Elimination of out of focus planes, yielding sharper images than conventional fluorescence microscopy.
- Analysis of thick specimens.
- Computer controlled microscopy, permitting fine focus control and digitization of images.
- Collection of data sets for subsequent three dimensional reconstruction.
- Ability to collect two images simultaneously from specimens labeled with multiple fluorochromes.
Confocal laser scanning microscopy permits high resolution optical sectioning without compromising specimen integrity. Some advantages of using confocal microscopy include:
- Elimination of out of focus planes, yielding sharper images than conventional fluorescence microscopy.
- Analysis of thick specimens.
- Computer controlled microscopy, permitting fine focus control and digitization of images.
- Collection of data sets for subsequent three dimensional reconstruction.
- Ability to collect two images simultaneously from specimens labeled with multiple fluorochromes.
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