A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells

Randolph J. Noelle; Meenakshi Roy; David M. Shepherd; Ivan Stamenkovico; Jeffrey A. Ledbetter; Alejandro Aruffo

A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells

Randolph J. Noelle, Meenakshi Roy, David M. Shepherd, Ivan Stamenkovico, Jeffrey A. Ledbetter, Alejandro Aruffo

Research output: Contribution to journal › Article › peer-review

Abstract

CD40 is a B-cell surface molecule that has been shown to induce B-cell growth upon ligation with monoclonal antibodies. This report shows that triggering via CD40 is essential for the activation of resting B cells by helper T cells (T_h). A soluble fusion protein of CD40 and human immunoglobulin, CD40-Ig, inhibited tbe induction of B-cell cycle entry, proliferation, and differentiation by activated T_h1 and T_h2. The ligand for CD40 was identified as a 39-kDa membrane protein that was selectively expressed on activated T_h. A monoclonal antibody specific for tbe 39-kDa protein inhibited CEMO-Ig binding and also inhibited the activation of B cells by T_h. These data indicate that the 39-kDa membrane protein expressed on activated T_h is a binding protein for CD40 and functions to transduce the signal for T_h-depcndenl B-cell activation.

Original language	English
Pages (from-to)	4195-4199
Number of pages	5
Journal	Journal of Immunology
Volume	197
Issue number	11
State	Published - Dec 1 2016

Cite this

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title = "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells",

abstract = "CD40 is a B-cell surface molecule that has been shown to induce B-cell growth upon ligation with monoclonal antibodies. This report shows that triggering via CD40 is essential for the activation of resting B cells by helper T cells (Th). A soluble fusion protein of CD40 and human immunoglobulin, CD40-Ig, inhibited tbe induction of B-cell cycle entry, proliferation, and differentiation by activated Th1 and Th2. The ligand for CD40 was identified as a 39-kDa membrane protein that was selectively expressed on activated Th. A monoclonal antibody specific for tbe 39-kDa protein inhibited CEMO-Ig binding and also inhibited the activation of B cells by Th. These data indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-depcndenl B-cell activation.",

author = "Noelle, {Randolph J.} and Meenakshi Roy and Shepherd, {David M.} and Ivan Stamenkovico and Ledbetter, {Jeffrey A.} and Alejandro Aruffo",

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language = "English",

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T1 - A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells

AU - Noelle, Randolph J.

AU - Roy, Meenakshi

AU - Shepherd, David M.

AU - Stamenkovico, Ivan

AU - Ledbetter, Jeffrey A.

AU - Aruffo, Alejandro

PY - 2016/12/1

Y1 - 2016/12/1

N2 - CD40 is a B-cell surface molecule that has been shown to induce B-cell growth upon ligation with monoclonal antibodies. This report shows that triggering via CD40 is essential for the activation of resting B cells by helper T cells (Th). A soluble fusion protein of CD40 and human immunoglobulin, CD40-Ig, inhibited tbe induction of B-cell cycle entry, proliferation, and differentiation by activated Th1 and Th2. The ligand for CD40 was identified as a 39-kDa membrane protein that was selectively expressed on activated Th. A monoclonal antibody specific for tbe 39-kDa protein inhibited CEMO-Ig binding and also inhibited the activation of B cells by Th. These data indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-depcndenl B-cell activation.

AB - CD40 is a B-cell surface molecule that has been shown to induce B-cell growth upon ligation with monoclonal antibodies. This report shows that triggering via CD40 is essential for the activation of resting B cells by helper T cells (Th). A soluble fusion protein of CD40 and human immunoglobulin, CD40-Ig, inhibited tbe induction of B-cell cycle entry, proliferation, and differentiation by activated Th1 and Th2. The ligand for CD40 was identified as a 39-kDa membrane protein that was selectively expressed on activated Th. A monoclonal antibody specific for tbe 39-kDa protein inhibited CEMO-Ig binding and also inhibited the activation of B cells by Th. These data indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-depcndenl B-cell activation.

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M3 - Article

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JO - Journal of Immunology

JF - Journal of Immunology

IS - 11

ER -

A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells

Abstract

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