TY - JOUR
T1 - A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells
AU - Noelle, Randolph J.
AU - Roy, Meenakshi
AU - Shepherd, David M.
AU - Stamenkovic, Ivan
AU - Ledbetter, Jeffrey A.
AU - Aruffo, Alejandro
PY - 1992/7/15
Y1 - 1992/7/15
N2 - CD40 is a B-cell surface molecule that has been shown to induce B-cell growth upon ligation with monoclonal antibodies. This report shows that triggering via CD40 is essential for the activation of resting B cells by helper T cells (Th). A soluble fusion protein of CD40 and human immunoglobulin, CD40-Ig, inhibited the induction of B-cell cycle entry, proliferation, and differentiation by activated Th1 and Th2. The ligand for CD40 was identified as a 39-kDa membrane protein that was selectively expressed on activated Th. A monoclonal antibody specific for the 39-kDa protein inhibited CD40-Ig binding and also inhibited the activation of B cells by Th. These data indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-dependent B-cell activation.
AB - CD40 is a B-cell surface molecule that has been shown to induce B-cell growth upon ligation with monoclonal antibodies. This report shows that triggering via CD40 is essential for the activation of resting B cells by helper T cells (Th). A soluble fusion protein of CD40 and human immunoglobulin, CD40-Ig, inhibited the induction of B-cell cycle entry, proliferation, and differentiation by activated Th1 and Th2. The ligand for CD40 was identified as a 39-kDa membrane protein that was selectively expressed on activated Th. A monoclonal antibody specific for the 39-kDa protein inhibited CD40-Ig binding and also inhibited the activation of B cells by Th. These data indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-dependent B-cell activation.
UR - http://www.scopus.com/inward/record.url?scp=0026772133&partnerID=8YFLogxK
U2 - 10.1073/pnas.89.14.6550
DO - 10.1073/pnas.89.14.6550
M3 - Article
C2 - 1378631
AN - SCOPUS:0026772133
SN - 0027-8424
VL - 89
SP - 6550
EP - 6554
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 14
ER -