A blocking primer increases specificity in environmental DNA detection of bull trout (Salvelinus confluentus)

Taylor M. Wilcox, Michael K. Schwartz, Kevin S. McKelvey, Michael K. Young, Winsor H. Lowe

Research output: Contribution to journalArticlepeer-review

Abstract

Environmental DNA (eDNA) is increasingly applied as a highly sensitive way to detect aquatic animals non-invasively. However, distinguishing closely related taxa can be particularly challenging. Previous studies of ancient DNA and genetic diet analysis have used blocking primers to enrich target template in the presence of abundant, non-target DNA. Here we apply a blocking primer to increase the specificity of a TaqMan assay for eDNA detection of rare and endangered bull trout (Salvelinus confluentus) in the presence of the closely related (Salvelinus namaycush). We found that addition of a blocking primer substantially increased assay specificity without compromising sensitivity or quantification ability.

Original languageEnglish
Pages (from-to)283-284
Number of pages2
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
StatePublished - Jun 2014

Keywords

  • Blocking primer
  • Bull trout
  • Complex templates
  • Environmental DNA

Fingerprint

Dive into the research topics of 'A blocking primer increases specificity in environmental DNA detection of bull trout (Salvelinus confluentus)'. Together they form a unique fingerprint.

Cite this