A fungal endophyte-tree relationship: Phoma sp. in Taxus wallachiana

Xianshu Yang; Gary Strobel; Andrea Stierle; W. M. Hess; Julie Lee; Jon Clardy

doi:10.1016/0168-9452(94)90017-5

A fungal endophyte-tree relationship: Phoma sp. in Taxus wallachiana

Xianshu Yang, Gary Strobel, Andrea Stierle, W. M. Hess, Julie Lee, Jon Clardy

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Taxus wallachiana, Nepalase yew, has many associated endophytic fungi. One of these is a Phoma sp. that lives primarily in the intercellular spaces of tissues in the phloem/cambial region of the tree limbs producing two antibiotic substances: altersolanol A and 2-hydroxy-6-methylbenzoic acid. These compounds were isolated from fungal culture, crystallized, bioassayed, and structurally characterized by X-ray crystallography. The presence of endophytic fungi raises the question as to their role in the tree, however, we suggest that the T. wallachiana/Phoma sp. relationship is a mutualistic one. Normally, the organic solvent extract of yew is not antibacterial, however the organic solvent extract of T. media c.v. Hicksii, inoculated with Phoma sp., possessed antibacterial activity. This extract contains altersolanol A as demonstrated by chromatographic and mass spectroscopic analyses.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Plant Science
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0168-9452(94)90017-5
State	Published - 1994

Keywords

2-Hydroxy-6-methylbenzoic acid
Altersolanol
Antibiotics
Endophytes
Mutualism
Yews

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10.1016/0168-9452(94)90017-5

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@article{54247316d48949c782ea113acfff6b39,

title = "A fungal endophyte-tree relationship: Phoma sp. in Taxus wallachiana",

abstract = "Taxus wallachiana, Nepalase yew, has many associated endophytic fungi. One of these is a Phoma sp. that lives primarily in the intercellular spaces of tissues in the phloem/cambial region of the tree limbs producing two antibiotic substances: altersolanol A and 2-hydroxy-6-methylbenzoic acid. These compounds were isolated from fungal culture, crystallized, bioassayed, and structurally characterized by X-ray crystallography. The presence of endophytic fungi raises the question as to their role in the tree, however, we suggest that the T. wallachiana/Phoma sp. relationship is a mutualistic one. Normally, the organic solvent extract of yew is not antibacterial, however the organic solvent extract of T. media c.v. Hicksii, inoculated with Phoma sp., possessed antibacterial activity. This extract contains altersolanol A as demonstrated by chromatographic and mass spectroscopic analyses.",

keywords = "2-Hydroxy-6-methylbenzoic acid, Altersolanol, Antibiotics, Endophytes, Mutualism, Yews",

author = "Xianshu Yang and Gary Strobel and Andrea Stierle and Hess, {W. M.} and Julie Lee and Jon Clardy",

note = "Funding Information: The authors thank the Royal Nepalese Academy of Sciences, Dr. B. Bashyal and others for helping to acquire T. wallachiana in North Central Nepal. Dr. Joe Sears kindly did the mass spectroscopy reported herein. Mr. Mike Standing assisted with the electron and light microscopy. Ms. Suzan Strobel provided the hand drawing of the fungal pseudosclerotia. Dr. B. Sutton, of the CMI in England, confirmed the identity of the fungus. Financial support for this project was provided by the NCI, the Montana Science and Tech Alliance, and the Montana Agricultural Experiment Station.",

year = "1994",

doi = "10.1016/0168-9452(94)90017-5",

language = "English",

volume = "102",

pages = "1--9",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier Ireland Ltd",

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T1 - A fungal endophyte-tree relationship

T2 - Phoma sp. in Taxus wallachiana

AU - Yang, Xianshu

AU - Strobel, Gary

AU - Stierle, Andrea

AU - Hess, W. M.

AU - Lee, Julie

AU - Clardy, Jon

N1 - Funding Information: The authors thank the Royal Nepalese Academy of Sciences, Dr. B. Bashyal and others for helping to acquire T. wallachiana in North Central Nepal. Dr. Joe Sears kindly did the mass spectroscopy reported herein. Mr. Mike Standing assisted with the electron and light microscopy. Ms. Suzan Strobel provided the hand drawing of the fungal pseudosclerotia. Dr. B. Sutton, of the CMI in England, confirmed the identity of the fungus. Financial support for this project was provided by the NCI, the Montana Science and Tech Alliance, and the Montana Agricultural Experiment Station.

PY - 1994

Y1 - 1994

N2 - Taxus wallachiana, Nepalase yew, has many associated endophytic fungi. One of these is a Phoma sp. that lives primarily in the intercellular spaces of tissues in the phloem/cambial region of the tree limbs producing two antibiotic substances: altersolanol A and 2-hydroxy-6-methylbenzoic acid. These compounds were isolated from fungal culture, crystallized, bioassayed, and structurally characterized by X-ray crystallography. The presence of endophytic fungi raises the question as to their role in the tree, however, we suggest that the T. wallachiana/Phoma sp. relationship is a mutualistic one. Normally, the organic solvent extract of yew is not antibacterial, however the organic solvent extract of T. media c.v. Hicksii, inoculated with Phoma sp., possessed antibacterial activity. This extract contains altersolanol A as demonstrated by chromatographic and mass spectroscopic analyses.

AB - Taxus wallachiana, Nepalase yew, has many associated endophytic fungi. One of these is a Phoma sp. that lives primarily in the intercellular spaces of tissues in the phloem/cambial region of the tree limbs producing two antibiotic substances: altersolanol A and 2-hydroxy-6-methylbenzoic acid. These compounds were isolated from fungal culture, crystallized, bioassayed, and structurally characterized by X-ray crystallography. The presence of endophytic fungi raises the question as to their role in the tree, however, we suggest that the T. wallachiana/Phoma sp. relationship is a mutualistic one. Normally, the organic solvent extract of yew is not antibacterial, however the organic solvent extract of T. media c.v. Hicksii, inoculated with Phoma sp., possessed antibacterial activity. This extract contains altersolanol A as demonstrated by chromatographic and mass spectroscopic analyses.

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A fungal endophyte-tree relationship: Phoma sp. in Taxus wallachiana

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Keywords

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