A highly repetitive DNA sequence possibly unique to canids

Michael F. Minnick; Lisa C. Stillwell; Jill M. Heineman; Gary L. Stiegler

doi:10.1016/0378-1119(92)90654-8

A highly repetitive DNA sequence possibly unique to canids

Michael F. Minnick, Lisa C. Stillwell, Jill M. Heineman, Gary L. Stiegler

Biological Sciences

Pacific Northwest National Laboratory

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A short interspersed nucleotide (nt) element (SINE) was cloned from the genomic DNA of the domestic dog, Canis familiaris. Southern-blot analysis of canine DNA digested with four restriction endonucleases indicated that the SINE is widely dispersed throughout the genome. Hybridization also indicated that the element may be unique to canids and is absent in a variety of other mammals, including members of four closely-related carnivore families. Three examples of the SINE have been located and sequenced. The 130-bp SINE contains putative RNA polymerase III transcriptional control sequences. The SINE is flanked at the 3′ end by a (TC)₈-repeat region followed by a poly(A) tract of 35-65 nt. Computer database searches located two homologous sequences with approx. 80% identity to the SINE. These sequences were located in untranslated regions of the canine genes encoding interferon-ω and clotting factor IX.

Original language	English
Pages (from-to)	235-238
Number of pages	4
Journal	Gene
Volume	110
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(92)90654-8
State	Published - Jan 15 1992

Keywords

Canis familiaris
dog
interferon
nucleotide sequence
repetitive element
short interspersed nucleotide element

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10.1016/0378-1119(92)90654-8

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title = "A highly repetitive DNA sequence possibly unique to canids",

abstract = "A short interspersed nucleotide (nt) element (SINE) was cloned from the genomic DNA of the domestic dog, Canis familiaris. Southern-blot analysis of canine DNA digested with four restriction endonucleases indicated that the SINE is widely dispersed throughout the genome. Hybridization also indicated that the element may be unique to canids and is absent in a variety of other mammals, including members of four closely-related carnivore families. Three examples of the SINE have been located and sequenced. The 130-bp SINE contains putative RNA polymerase III transcriptional control sequences. The SINE is flanked at the 3′ end by a (TC)8-repeat region followed by a poly(A) tract of 35-65 nt. Computer database searches located two homologous sequences with approx. 80% identity to the SINE. These sequences were located in untranslated regions of the canine genes encoding interferon-ω and clotting factor IX.",

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T1 - A highly repetitive DNA sequence possibly unique to canids

AU - Minnick, Michael F.

AU - Stillwell, Lisa C.

AU - Heineman, Jill M.

AU - Stiegler, Gary L.

PY - 1992/1/15

Y1 - 1992/1/15

N2 - A short interspersed nucleotide (nt) element (SINE) was cloned from the genomic DNA of the domestic dog, Canis familiaris. Southern-blot analysis of canine DNA digested with four restriction endonucleases indicated that the SINE is widely dispersed throughout the genome. Hybridization also indicated that the element may be unique to canids and is absent in a variety of other mammals, including members of four closely-related carnivore families. Three examples of the SINE have been located and sequenced. The 130-bp SINE contains putative RNA polymerase III transcriptional control sequences. The SINE is flanked at the 3′ end by a (TC)8-repeat region followed by a poly(A) tract of 35-65 nt. Computer database searches located two homologous sequences with approx. 80% identity to the SINE. These sequences were located in untranslated regions of the canine genes encoding interferon-ω and clotting factor IX.

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KW - Canis familiaris

KW - dog

KW - interferon

KW - nucleotide sequence

KW - repetitive element

KW - short interspersed nucleotide element

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JO - Gene

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A highly repetitive DNA sequence possibly unique to canids

Abstract

Keywords

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