We studied 60 patients with non-small cell lung cancer (NSCLC) for alterations in the epidermal growth factor receptor (EGFR) gene. 24 60 (40%) demonstrated cleavage of a 4.2 kb EcoRI fragment in the tumor DNA which was not observed in DNA from paired normal lung. This fragment corresponds to a genomic region that encodes for part of the intracellular tyrosine kinase domain of the EGFR. We demonstrated that an alteration is present which leads to an increased cleavage efficiency of restriction endonuclease EcoRI for a specific EcoRI site in this 4.2 kb fragment.
- Epidermal growth factor receptor
- Lung cancer