A novel delta-endotoxin gene cryIM from Bacillus thuringiensis ssp. wuhanensis

A. B. Shevelev, Ya N. Kogan, A. M. Bushueva, E. J. Voronina, D. V. Rebrikov, S. I. Novikova, G. G. Chestukhina, V. Kuvshinov, E. Pehu, V. M. Stepanov

Research output: Contribution to journalArticlepeer-review

Abstract

A new cryI-related sequence designated cryIM was cloned using an immunoscreening technique from ssp. wuhanensis of Bacillus thuringiensis (BT), previously reported to produce multiple Cry proteins [Chestukhina et al. (1994) Can. J. Microbiol. 240, 1026-1034]. Analysis of the cryIM nucleotide sequence revealed an ORF, BTII-type promoter-like sequence, peculiar for such genes, a translation initiation element and a putative transcription terminator. Nevertheless, its product was not previously found in the crystals of the host strain [Chestukhina et al. (1994) Can. J. Microbiol. 240, 1026-1034] which shows its weak or absent natural expression. The amino acid sequence of 1151 residues encoded by the continuous reading frame of cryIM is not identical but is essentially similar to the other δ-endotoxlns of the CryI class. An IS231-like sequence was found 400 bp downstream of the cryIM stop codon and a fragment of the cryIAb gene was located 3 kb upstream of its initiator codon in the same orientation. Artificial expression of the cloned gene in E. coli under the control of the lacZ promoter allowed us to obtain its hypothetical protein product.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
StatePublished - Mar 10 1997

Keywords

  • Bacillus thuringiensis
  • CryIAb
  • CryIM
  • Delta-endotoxin
  • IS231
  • Primary structure

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