@article{687b913b5a9942f1bcd81424b6f76249,
title = "Adjustable ellipsoid nanoparticles assembled from re-engineered connectors of the bacteriophage Phi29 DNA packaging motor",
abstract = "A 24 × 30 nm ellipsoid nanoparticle containing 84 subunits or 7 dodecamers of the re-engineered core protein of the bacteriophage phi29 packaging motor was constructed. Homogeneous nanoparticles were obtained with simple one-step purification. Electron microscopy and analytical ultracentrifugation were employed to elucidate the structure, shape, size, and mechanism of assembly. The formation of this structure was mediated and stabilized by N-terminal peptide extensions. Reversal of the 84-subunit ellipsoid nanoparticle to its dodecamer subunit was controlled by the cleavage of the extended N-terminal peptide with a protease. The 84 outward-oriented C-termini were conjugated with a streptavidin binding peptide which can be used for the incorporation of markers. This further extends the application of this nanoparticle to pathogen detection and disease diagnosis by signal enhancement.",
keywords = "Bacteriophage phi29 connector, Bionanotechnology, Nanobiotechnology, Phi29 DNA packaging motor, Protein nanoparticles, Viral DNA packaging, Virus assemble",
author = "Feng Xiao and Ying Cai and Wang, \{Joseph Che Yen\} and Dominik Green and Cheng, \{R. Holland\} and Borries Demeler and Peixuan Guo",
year = "2009",
month = aug,
day = "25",
doi = "10.1021/nn900187k",
language = "English",
volume = "3",
pages = "2163--2170",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "8",
}