Analysis of strain-specific plasmid sequences from Coxiella burnetii.

M. F. Minnick; C. L. Small; M. E. Frazier; L. P. Mallavia

Analysis of strain-specific plasmid sequences from Coxiella burnetii.

M. F. Minnick, C. L. Small, M. E. Frazier, L. P. Mallavia

Biological Sciences

Washington State University Pullman

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Acute isolates of Coxiella burnetii possess a 36-kbp plasmid termed QpH1. DNA hybridizations show that QpH1 contains approximately 6-kbp region of DNA which is not present in the QpRS plasmid from chronic isolates. This QpH1-specific region of DNA contains the contiguous EcoRI fragments G, E, and D. The GED region was found to possess seven open reading frames (ORF's) coding for proteins ranging from 5.5 to 42.3 kDa in molecular mass when subcloned and expressed in vitro. Summing the predicted ORF's accounts for 95% of the GED coding potential. E. coli expression produced a stable 42.3-kDa protein from the pHIN19 subclone of GED. The ORF of the 42.3-kDa protein, termed cbhE', has been localized on GED by both in vitro transcription/translation and DNA sequencing. The cbhE' gene is estimated as 1142 bp in length with a putative promoter region of TCAACT (-35)-N16-TAAAAT (-10)-N14-AGAAGGA (Shine-Dalgarno)-N10-ATG.

Original language	English
Pages (from-to)	497-502
Number of pages	6
Journal	Acta Virologica
Volume	35
Issue number	6
State	Published - Nov 1991

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@article{44e5a346396342b1af47a52d1b890707,

title = "Analysis of strain-specific plasmid sequences from Coxiella burnetii.",

abstract = "Acute isolates of Coxiella burnetii possess a 36-kbp plasmid termed QpH1. DNA hybridizations show that QpH1 contains approximately 6-kbp region of DNA which is not present in the QpRS plasmid from chronic isolates. This QpH1-specific region of DNA contains the contiguous EcoRI fragments G, E, and D. The GED region was found to possess seven open reading frames (ORF's) coding for proteins ranging from 5.5 to 42.3 kDa in molecular mass when subcloned and expressed in vitro. Summing the predicted ORF's accounts for 95% of the GED coding potential. E. coli expression produced a stable 42.3-kDa protein from the pHIN19 subclone of GED. The ORF of the 42.3-kDa protein, termed cbhE', has been localized on GED by both in vitro transcription/translation and DNA sequencing. The cbhE' gene is estimated as 1142 bp in length with a putative promoter region of TCAACT (-35)-N16-TAAAAT (-10)-N14-AGAAGGA (Shine-Dalgarno)-N10-ATG.",

author = "Minnick, {M. F.} and Small, {C. L.} and Frazier, {M. E.} and Mallavia, {L. P.}",

year = "1991",

month = nov,

language = "English",

volume = "35",

pages = "497--502",

journal = "Acta Virologica",

issn = "0001-723X",

publisher = "Frontiers Media SA",

number = "6",

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TY - JOUR

T1 - Analysis of strain-specific plasmid sequences from Coxiella burnetii.

AU - Minnick, M. F.

AU - Small, C. L.

AU - Frazier, M. E.

AU - Mallavia, L. P.

PY - 1991/11

Y1 - 1991/11

N2 - Acute isolates of Coxiella burnetii possess a 36-kbp plasmid termed QpH1. DNA hybridizations show that QpH1 contains approximately 6-kbp region of DNA which is not present in the QpRS plasmid from chronic isolates. This QpH1-specific region of DNA contains the contiguous EcoRI fragments G, E, and D. The GED region was found to possess seven open reading frames (ORF's) coding for proteins ranging from 5.5 to 42.3 kDa in molecular mass when subcloned and expressed in vitro. Summing the predicted ORF's accounts for 95% of the GED coding potential. E. coli expression produced a stable 42.3-kDa protein from the pHIN19 subclone of GED. The ORF of the 42.3-kDa protein, termed cbhE', has been localized on GED by both in vitro transcription/translation and DNA sequencing. The cbhE' gene is estimated as 1142 bp in length with a putative promoter region of TCAACT (-35)-N16-TAAAAT (-10)-N14-AGAAGGA (Shine-Dalgarno)-N10-ATG.

AB - Acute isolates of Coxiella burnetii possess a 36-kbp plasmid termed QpH1. DNA hybridizations show that QpH1 contains approximately 6-kbp region of DNA which is not present in the QpRS plasmid from chronic isolates. This QpH1-specific region of DNA contains the contiguous EcoRI fragments G, E, and D. The GED region was found to possess seven open reading frames (ORF's) coding for proteins ranging from 5.5 to 42.3 kDa in molecular mass when subcloned and expressed in vitro. Summing the predicted ORF's accounts for 95% of the GED coding potential. E. coli expression produced a stable 42.3-kDa protein from the pHIN19 subclone of GED. The ORF of the 42.3-kDa protein, termed cbhE', has been localized on GED by both in vitro transcription/translation and DNA sequencing. The cbhE' gene is estimated as 1142 bp in length with a putative promoter region of TCAACT (-35)-N16-TAAAAT (-10)-N14-AGAAGGA (Shine-Dalgarno)-N10-ATG.

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M3 - Article

C2 - 1687630

AN - SCOPUS:0026251265

SN - 0001-723X

VL - 35

SP - 497

EP - 502

JO - Acta Virologica

JF - Acta Virologica

IS - 6

ER -

Analysis of strain-specific plasmid sequences from Coxiella burnetii.

Abstract

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