Analysis of the cbhE' plasmid gene from acute disease-causing isolates of Coxiella burnetii

Michael F. Minnick; Christopher L. Small; Marvin E. Frazier; Louis P. Mallavia

doi:10.1016/0378-1119(91)90401-V

Analysis of the cbhE' plasmid gene from acute disease-causing isolates of Coxiella burnetii

Michael F. Minnick, Christopher L. Small, Marvin E. Frazier, Louis P. Mallavia

Biological Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A gene termed cbhE' was cloned from the QpH 1 plasmid of Coxiella burnetii. Expression of recombinants containing cbhE' in vitro and in Escherichia coli maxicells, produced an insert-encoded polypeptide of approx. 42 kDa. The CbhE protein was not cleaved when intact maxicells were treated with trypsin. Hybridizations of total DNA isolated from the six strains of C. burnetii indicate that this gene is unique to C. burnetii strains associated with acute disease i.e., Hamilton[I], Vacca[II], and Rasche[III]. The cbhE' gene was not detected in strains associated with chronic disease (Biotzere[IV] and Corazon[V]) or the Dod[VI] strain. The cbhE' open reading frame (ORF) is 1022 bp in length and is preceded by a predicted promoter/Shine-Dalgarno (SD) region of TCAACT (-35)-N₁₆-TAAAAT (-10) -N₁₄-AGAAGGA (SD) located 10 nucleotides (nt) before the presumed AUG start codon. The ORF ends with a single UAA stop codon and has no apparent Rho-factor-independent terminator following it. The cbhE' gene codes for the CbhE protein of 341 amino acid (aa) residues with a deduced M_r of 39442. CbhE is predominantly hydrophilic with a predicted pI of 4.43. The function of CbhE is unknown. No nt or aa sequences with homology to cbhE' or CbhE, respectively, were found in searches of a number of data bases.

Original language	English
Pages (from-to)	113-118
Number of pages	6
Journal	Gene
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(91)90401-V
State	Published - Jul 15 1991

Keywords

Q fever
QpH1
QpRS
Recombinant DNA
chronic disease
medical microbiology
rickettsiae, nucleotide sequence

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10.1016/0378-1119(91)90401-V

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@article{9463d26abe3f479787604637ba243c06,

title = "Analysis of the cbhE' plasmid gene from acute disease-causing isolates of Coxiella burnetii",

abstract = "A gene termed cbhE' was cloned from the QpH 1 plasmid of Coxiella burnetii. Expression of recombinants containing cbhE' in vitro and in Escherichia coli maxicells, produced an insert-encoded polypeptide of approx. 42 kDa. The CbhE protein was not cleaved when intact maxicells were treated with trypsin. Hybridizations of total DNA isolated from the six strains of C. burnetii indicate that this gene is unique to C. burnetii strains associated with acute disease i.e., Hamilton[I], Vacca[II], and Rasche[III]. The cbhE' gene was not detected in strains associated with chronic disease (Biotzere[IV] and Corazon[V]) or the Dod[VI] strain. The cbhE' open reading frame (ORF) is 1022 bp in length and is preceded by a predicted promoter/Shine-Dalgarno (SD) region of TCAACT (-35)-N16-TAAAAT (-10) -N14-AGAAGGA (SD) located 10 nucleotides (nt) before the presumed AUG start codon. The ORF ends with a single UAA stop codon and has no apparent Rho-factor-independent terminator following it. The cbhE' gene codes for the CbhE protein of 341 amino acid (aa) residues with a deduced Mr of 39442. CbhE is predominantly hydrophilic with a predicted pI of 4.43. The function of CbhE is unknown. No nt or aa sequences with homology to cbhE' or CbhE, respectively, were found in searches of a number of data bases.",

keywords = "Q fever, QpH1, QpRS, Recombinant DNA, chronic disease, medical microbiology, rickettsiae, nucleotide sequence",

author = "Minnick, {Michael F.} and Small, {Christopher L.} and Frazier, {Marvin E.} and Mallavia, {Louis P.}",

note = "Funding Information: We express our gratitude to Shirley Schmitt and Ellen Sisk for excellent technicaals sistance, to the VADAMS laboratory of Washington State University for providing sequence analysis programs, and to the Laboratory for Biotechnology and Bioanalysis of Washington State University for providing the oligo primers. This work was supported by grant AI20190 from the National Institutes of Health (NIAID) and by Battelle, PNL under contract No. DEAC06 76RLD 1831.",

year = "1991",

month = jul,

day = "15",

doi = "10.1016/0378-1119(91)90401-V",

language = "English",

volume = "103",

pages = "113--118",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1",

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T1 - Analysis of the cbhE' plasmid gene from acute disease-causing isolates of Coxiella burnetii

AU - Minnick, Michael F.

AU - Small, Christopher L.

AU - Frazier, Marvin E.

AU - Mallavia, Louis P.

N1 - Funding Information: We express our gratitude to Shirley Schmitt and Ellen Sisk for excellent technicaals sistance, to the VADAMS laboratory of Washington State University for providing sequence analysis programs, and to the Laboratory for Biotechnology and Bioanalysis of Washington State University for providing the oligo primers. This work was supported by grant AI20190 from the National Institutes of Health (NIAID) and by Battelle, PNL under contract No. DEAC06 76RLD 1831.

PY - 1991/7/15

Y1 - 1991/7/15

N2 - A gene termed cbhE' was cloned from the QpH 1 plasmid of Coxiella burnetii. Expression of recombinants containing cbhE' in vitro and in Escherichia coli maxicells, produced an insert-encoded polypeptide of approx. 42 kDa. The CbhE protein was not cleaved when intact maxicells were treated with trypsin. Hybridizations of total DNA isolated from the six strains of C. burnetii indicate that this gene is unique to C. burnetii strains associated with acute disease i.e., Hamilton[I], Vacca[II], and Rasche[III]. The cbhE' gene was not detected in strains associated with chronic disease (Biotzere[IV] and Corazon[V]) or the Dod[VI] strain. The cbhE' open reading frame (ORF) is 1022 bp in length and is preceded by a predicted promoter/Shine-Dalgarno (SD) region of TCAACT (-35)-N16-TAAAAT (-10) -N14-AGAAGGA (SD) located 10 nucleotides (nt) before the presumed AUG start codon. The ORF ends with a single UAA stop codon and has no apparent Rho-factor-independent terminator following it. The cbhE' gene codes for the CbhE protein of 341 amino acid (aa) residues with a deduced Mr of 39442. CbhE is predominantly hydrophilic with a predicted pI of 4.43. The function of CbhE is unknown. No nt or aa sequences with homology to cbhE' or CbhE, respectively, were found in searches of a number of data bases.

AB - A gene termed cbhE' was cloned from the QpH 1 plasmid of Coxiella burnetii. Expression of recombinants containing cbhE' in vitro and in Escherichia coli maxicells, produced an insert-encoded polypeptide of approx. 42 kDa. The CbhE protein was not cleaved when intact maxicells were treated with trypsin. Hybridizations of total DNA isolated from the six strains of C. burnetii indicate that this gene is unique to C. burnetii strains associated with acute disease i.e., Hamilton[I], Vacca[II], and Rasche[III]. The cbhE' gene was not detected in strains associated with chronic disease (Biotzere[IV] and Corazon[V]) or the Dod[VI] strain. The cbhE' open reading frame (ORF) is 1022 bp in length and is preceded by a predicted promoter/Shine-Dalgarno (SD) region of TCAACT (-35)-N16-TAAAAT (-10) -N14-AGAAGGA (SD) located 10 nucleotides (nt) before the presumed AUG start codon. The ORF ends with a single UAA stop codon and has no apparent Rho-factor-independent terminator following it. The cbhE' gene codes for the CbhE protein of 341 amino acid (aa) residues with a deduced Mr of 39442. CbhE is predominantly hydrophilic with a predicted pI of 4.43. The function of CbhE is unknown. No nt or aa sequences with homology to cbhE' or CbhE, respectively, were found in searches of a number of data bases.

KW - Q fever

KW - QpH1

KW - QpRS

KW - Recombinant DNA

KW - chronic disease

KW - medical microbiology

KW - rickettsiae, nucleotide sequence

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U2 - 10.1016/0378-1119(91)90401-V

DO - 10.1016/0378-1119(91)90401-V

M3 - Article

C2 - 1879692

AN - SCOPUS:0025868307

SN - 0378-1119

VL - 103

SP - 113

EP - 118

JO - Gene

JF - Gene

IS - 1

ER -

Analysis of the cbhE' plasmid gene from acute disease-causing isolates of Coxiella burnetii

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Keywords

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