TY - JOUR
T1 - Anti-inflammatory effects of natural product formulations on murine macrophages
AU - Benson, Jenna M.
AU - Miller, Andrea K.
AU - Cooper, Natalie
AU - Muanza, Dave N.
AU - Smith, Jerry R.
AU - Shepherd, David M.
N1 - Funding Information:
Jenna M. Benson is affiliated with the Center for Environmental Health Sciences, Department of Biomedical and Pharmaceutical Sciences, University of Montana, Montana, USA. Andrea K. Miller is affiliated with the Center for Environmental Health Sciences, University of Montana, Montana, USA. Natalie Cooper is affiliated with the Skaggs School of Pharmacy, University of Montana, Montana, USA. Dave N. Muanza is affiliated with the Phytochemistry Laboratory, Biotics Research Corporation, Rosenberg, Texas, USA. Jerry R. Smith is affiliated with the Department of Biomedical and Pharmaceutical Sciences, University of Montana, Montana, USA. David M. Shepherd is affiliated with the Center for Environmental Health Sciences, Department of Biomedical and Pharmaceutical Sciences, University of Montana, Montana, USA. Address correspondence to: David M. Shepherd, PhD, University of Montana, CEHS, 32 Campus Dr., 284 Skaggs, Missoula, MT 59812 (E-mail: [email protected]). This project was supported by the Biotics Research Corporation. The authors would like to acknowledge the assistance of the Fluorescence Cytometry Core Facility of the University of Montana supported in part by a grant from the NIH (P20RR017670-06).
PY - 2010/8
Y1 - 2010/8
N2 - The popularity of herbal supplements, especially those with purported anti-inflammatory effects, has drastically increased in recent years as more people have turned to natural therapeutics. As the supplement industry is loosely regulated, the safety and efficacy of these products is poorly understood. In the present study, we examined the effects of natural product formulations prepared by the Biotics Research Corporation (BRC) on cyclooxygenase (COX) enzyme activity. We also evaluated the immune responsiveness of RAW264.7 macrophages, a key cell population involved in the inflammation, to those formulations. As a result, three supplements, BRC-301, BRC-304, and BRC-306, selectively inhibited COX-2, the inducible isoform involved in inflammation. Further evaluation of these three products indicated that BRC-304 and BRC-306 produced minimal effects on the production of inflammatory mediators by lipopolysaccharide (LPS)-stimulated macrophages. BRC-301 decreased the LPS-induced production of nitric oxide and IL-6, as well as CD40 expression. Collectively, these results suggest that the BRC-301 extract, comprising several polyphenolic natural products, may have a protective effect in chronic inflammatory disorders.
AB - The popularity of herbal supplements, especially those with purported anti-inflammatory effects, has drastically increased in recent years as more people have turned to natural therapeutics. As the supplement industry is loosely regulated, the safety and efficacy of these products is poorly understood. In the present study, we examined the effects of natural product formulations prepared by the Biotics Research Corporation (BRC) on cyclooxygenase (COX) enzyme activity. We also evaluated the immune responsiveness of RAW264.7 macrophages, a key cell population involved in the inflammation, to those formulations. As a result, three supplements, BRC-301, BRC-304, and BRC-306, selectively inhibited COX-2, the inducible isoform involved in inflammation. Further evaluation of these three products indicated that BRC-304 and BRC-306 produced minimal effects on the production of inflammatory mediators by lipopolysaccharide (LPS)-stimulated macrophages. BRC-301 decreased the LPS-induced production of nitric oxide and IL-6, as well as CD40 expression. Collectively, these results suggest that the BRC-301 extract, comprising several polyphenolic natural products, may have a protective effect in chronic inflammatory disorders.
KW - Antigen presenting cells
KW - Dietary supplements
KW - Inflammation
KW - Macrophages
KW - Natural products
UR - http://www.scopus.com/inward/record.url?scp=77955582199&partnerID=8YFLogxK
U2 - 10.3109/19390211.2010.489035
DO - 10.3109/19390211.2010.489035
M3 - Article
C2 - 22432514
AN - SCOPUS:77955582199
SN - 1939-0211
VL - 7
SP - 227
EP - 239
JO - Journal of Dietary Supplements
JF - Journal of Dietary Supplements
IS - 3
ER -