Applications of lipid nanodiscs for the study of membrane proteins by surface plasmon resonance

Meg Trahey; Mavis Jiarong Li; Hyewon Kwon; Erica L. Woodahl; Wynton D. McClary; William M. Atkins

doi:10.1002/0471140864.ps2913s81

Applications of lipid nanodiscs for the study of membrane proteins by surface plasmon resonance

Meg Trahey, Mavis Jiarong Li, Hyewon Kwon, Erica L. Woodahl, Wynton D. McClary, William M. Atkins

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.

Original language	English
Pages (from-to)	29.13.1-29.13.16
Journal	Current Protocols in Protein Science
Volume	2015
DOIs	https://doi.org/10.1002/0471140864.ps2913s81
State	Published - 2015

Keywords

Antibody-receptor binding
Biosensor
Lipid nanodisc
Membrane protein
Surface plasmon resonance binding analysis

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10.1002/0471140864.ps2913s81

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abstract = "Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.",

keywords = "Antibody-receptor binding, Biosensor, Lipid nanodisc, Membrane protein, Surface plasmon resonance binding analysis",

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AU - Trahey, Meg

AU - Li, Mavis Jiarong

AU - Kwon, Hyewon

AU - Woodahl, Erica L.

AU - McClary, Wynton D.

AU - Atkins, William M.

PY - 2015

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AB - Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.

KW - Antibody-receptor binding

KW - Biosensor

KW - Lipid nanodisc

KW - Membrane protein

KW - Surface plasmon resonance binding analysis

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JO - Current Protocols in Protein Science

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Applications of lipid nanodiscs for the study of membrane proteins by surface plasmon resonance

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Keywords

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