Applications of lipid nanodiscs for the study of membrane proteins by surface plasmon resonance

Meg Trahey, Mavis Jiarong Li, Hyewon Kwon, Erica L. Woodahl, Wynton D. McClary, William M. Atkins

Research output: Contribution to journalArticlepeer-review

Abstract

Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.

Original languageEnglish
Pages (from-to)29.13.1-29.13.16
JournalCurrent Protocols in Protein Science
Volume2015
DOIs
StatePublished - 2015

Keywords

  • Antibody-receptor binding
  • Biosensor
  • Lipid nanodisc
  • Membrane protein
  • Surface plasmon resonance binding analysis

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