Applying 3D ED/MicroED workflows toward the next frontiers

Mahira Aragon; Sarah E.J. Bowman; Chun Hsing Chen; M. Jason de la Cruz; Daniel A. Decato; Edward T. Eng; Kristen M. Flatt; Sahil Gulati; Yuchen Li; Charles J. Lomba; Brandon Mercado; Jessalyn Miller; Lukáš Palatinus; William J. Rice; David Waterman; Christina M. Zimanyi

doi:10.1107/S2053229624004078

Applying 3D ED/MicroED workflows toward the next frontiers

Mahira Aragon
, Sarah E.J. Bowman
, Chun Hsing Chen
, M. Jason de la Cruz
, Daniel A. Decato
, Edward T. Eng
, Kristen M. Flatt
, Sahil Gulati
, Yuchen Li
, Charles J. Lomba
, Brandon Mercado
, Jessalyn Miller
, Lukáš Palatinus
, William J. Rice
, David Waterman
, Christina M. Zimanyi

Chemistry and Biochemistry

New York Structural Biology Center
Hauptman-Woodward Medical Research Institute, Inc.
University of North Carolina at Chapel Hill
Memorial Sloan-Kettering Cancer Center
University of Illinois at Urbana-Champaign
Gatan, Inc
Princeton University
Yale University
Czech Academy of Sciences
New York University
Rutherford Appleton Laboratory

Research output: Contribution to journal › Review article › peer-review

10 Scopus citations

Abstract

We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED), as discussed during a symposium at the National Center for CryoEM Access and Training housed at the New York Structural Biology Center. This snapshot describes cutting-edge developments in various facets of the field and identifies potential avenues for continued progress. Key sections discuss instrumentation access, research applications for small molecules and biomacromolecules, data collection hardware and software, data reduction software, and finally reporting and validation. 3D ED/MicroED is still early in its wide adoption by the structural science community with ample opportunities for expansion, growth, and innovation.

Original language	English
Pages (from-to)	179-189
Number of pages	11
Journal	Acta Crystallographica Section C: Structural Chemistry
Volume	80
Issue number	Pt 6
DOIs	https://doi.org/10.1107/S2053229624004078
State	Published - Jun 1 2024

Keywords

3D ED
electron diffraction
microcrystal electron diffraction
MicroED

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10.1107/S2053229624004078

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Aragon, M., Bowman, S. E. J., Chen, C. H., de la Cruz, M. J., Decato, D. A., Eng, E. T., Flatt, K. M., Gulati, S., Li, Y., Lomba, C. J., Mercado, B., Miller, J., Palatinus, L., Rice, W. J., Waterman, D., & Zimanyi, C. M. (2024). Applying 3D ED/MicroED workflows toward the next frontiers. Acta Crystallographica Section C: Structural Chemistry, 80(Pt 6), 179-189. https://doi.org/10.1107/S2053229624004078

@article{adf0f4705ab54885a89b6435069f779a,

title = "Applying 3D ED/MicroED workflows toward the next frontiers",

abstract = "We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED), as discussed during a symposium at the National Center for CryoEM Access and Training housed at the New York Structural Biology Center. This snapshot describes cutting-edge developments in various facets of the field and identifies potential avenues for continued progress. Key sections discuss instrumentation access, research applications for small molecules and biomacromolecules, data collection hardware and software, data reduction software, and finally reporting and validation. 3D ED/MicroED is still early in its wide adoption by the structural science community with ample opportunities for expansion, growth, and innovation.",

keywords = "3D ED, electron diffraction, microcrystal electron diffraction, MicroED",

author = "Mahira Aragon and Bowman, \{Sarah E.J.\} and Chen, \{Chun Hsing\} and \{de la Cruz\}, \{M. Jason\} and Decato, \{Daniel A.\} and Eng, \{Edward T.\} and Flatt, \{Kristen M.\} and Sahil Gulati and Yuchen Li and Lomba, \{Charles J.\} and Brandon Mercado and Jessalyn Miller and Luk{\'a}{\v s} Palatinus and Rice, \{William J.\} and David Waterman and Zimanyi, \{Christina M.\}",

year = "2024",

month = jun,

day = "1",

doi = "10.1107/S2053229624004078",

language = "English",

volume = "80",

pages = "179--189",

journal = "Acta Crystallographica Section C: Structural Chemistry",

issn = "1600-5759",

publisher = "John Wiley \& Sons Inc.",

number = "Pt 6",

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Aragon, M, Bowman, SEJ, Chen, CH, de la Cruz, MJ, Decato, DA, Eng, ET, Flatt, KM, Gulati, S, Li, Y, Lomba, CJ, Mercado, B, Miller, J, Palatinus, L, Rice, WJ, Waterman, D & Zimanyi, CM 2024, 'Applying 3D ED/MicroED workflows toward the next frontiers', Acta Crystallographica Section C: Structural Chemistry, vol. 80, no. Pt 6, pp. 179-189. https://doi.org/10.1107/S2053229624004078

TY - JOUR

T1 - Applying 3D ED/MicroED workflows toward the next frontiers

AU - Aragon, Mahira

AU - Bowman, Sarah E.J.

AU - Chen, Chun Hsing

AU - de la Cruz, M. Jason

AU - Decato, Daniel A.

AU - Eng, Edward T.

AU - Flatt, Kristen M.

AU - Gulati, Sahil

AU - Li, Yuchen

AU - Lomba, Charles J.

AU - Mercado, Brandon

AU - Miller, Jessalyn

AU - Palatinus, Lukáš

AU - Rice, William J.

AU - Waterman, David

AU - Zimanyi, Christina M.

PY - 2024/6/1

Y1 - 2024/6/1

N2 - We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED), as discussed during a symposium at the National Center for CryoEM Access and Training housed at the New York Structural Biology Center. This snapshot describes cutting-edge developments in various facets of the field and identifies potential avenues for continued progress. Key sections discuss instrumentation access, research applications for small molecules and biomacromolecules, data collection hardware and software, data reduction software, and finally reporting and validation. 3D ED/MicroED is still early in its wide adoption by the structural science community with ample opportunities for expansion, growth, and innovation.

AB - We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED), as discussed during a symposium at the National Center for CryoEM Access and Training housed at the New York Structural Biology Center. This snapshot describes cutting-edge developments in various facets of the field and identifies potential avenues for continued progress. Key sections discuss instrumentation access, research applications for small molecules and biomacromolecules, data collection hardware and software, data reduction software, and finally reporting and validation. 3D ED/MicroED is still early in its wide adoption by the structural science community with ample opportunities for expansion, growth, and innovation.

KW - 3D ED

KW - electron diffraction

KW - microcrystal electron diffraction

KW - MicroED

UR - https://www.scopus.com/pages/publications/85195327346

U2 - 10.1107/S2053229624004078

DO - 10.1107/S2053229624004078

M3 - Review article

C2 - 38712546

AN - SCOPUS:85195327346

SN - 1600-5759

VL - 80

SP - 179

EP - 189

JO - Acta Crystallographica Section C: Structural Chemistry

JF - Acta Crystallographica Section C: Structural Chemistry

IS - Pt 6

ER -

Applying 3D ED/MicroED workflows toward the next frontiers

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Keywords

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