Aryl hydrocarbon receptor (AhR) regulates silica-induced inflammation but not fibrosis

Celine A. Beamer, Benjamin P. Seaver, David M. Shepherd

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, is responsible for mediating a variety of pharmacological and toxicological effects caused by halogenated aromatic hydrocarbons such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). However, recent evidence has revealed that the AhR also has numerous physiological roles aside from xenobiotic metabolism, including regulation of immune and inflammatory signaling as well as normal development and homeostasis of several organs. To investigate the role of the AhR in crystalline silica (SiO2)-induced inflammation and fibrosis, C57Bl/6 and AhR-/- mice were exposed to SiO2 or vehicle. Similarly, C57Bl/6 mice were exposed to SiO2 and TCDD either simultaneously or sequentially to assess whether AhR activation alters inflammation and fibrosis. SiO2-induced acute lung inflammation was more severe in AhR-/- mice; however, the fibrotic response of AhR-/- mice was attenuated compared with C57Bl/6 mice. In a model of chronic SiO2 exposure, AhR activation by TCDD in C57Bl/6 mice resulted in reduced inflammation; however, the fibrotic response was not affected. Bone marrow-derived macrophages (BMM) from AhR-/- mice also produced higher levels of cytokines and chemokines in response to SiO2. Analysis of gene expression revealed that BMM derived from AhR-/- mice exhibit increased levels of pro-interleukin (IL)-1β, IL-6, and Bcl-2, yet decreased levels of signal transducers and activators of transcription (STAT)2, STAT5a, and serpin B2 (Pai-2) in response to SiO2.

Original languageEnglish
Pages (from-to)554-568
Number of pages15
JournalToxicological Sciences
Volume126
Issue number2
DOIs
StatePublished - Apr 2012

Keywords

  • Inflammasome
  • Interleukin 1β
  • Lung
  • Serpin B2 (Pai-2)

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