Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F2 mice

Veronica S. Valentinuzzi; Daniel E. Kolker; Martha Hotz Vitaterna; Kazuhiro Shimomura; Andrew Whiteley; Sharon Low-Zeddies; Fred W. Turek; Elenice A.M. Ferrari; Richard Paylor; Joseph S. Takahashi

doi:10.1101/lm.5.4.391

Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F₂ mice

Veronica S. Valentinuzzi, Daniel E. Kolker, Martha Hotz Vitaterna, Kazuhiro Shimomura, Andrew Whiteley, Sharon Low-Zeddies, Fred W. Turek, Elenice A.M. Ferrari, Richard Paylor, Joseph S. Takahashi

W. A. Franke College of Forestry and Conservation

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Abstract

The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F₂ mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test.

Original language	English
Pages (from-to)	391-403
Number of pages	13
Journal	Learning and Memory
Volume	5
Issue number	4-5
DOIs	https://doi.org/10.1101/lm.5.4.391
State	Published - 1998

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Valentinuzzi, V. S., Kolker, D. E., Vitaterna, M. H., Shimomura, K., Whiteley, A., Low-Zeddies, S., Turek, F. W., Ferrari, E. A. M., Paylor, R., & Takahashi, J. S. (1998). Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F₂ mice. Learning and Memory, 5(4-5), 391-403. https://doi.org/10.1101/lm.5.4.391

@article{b9ba1f269a6d4ef78d3f349f0830d5df,

title = "Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F2 mice",

abstract = "The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test.",

author = "Valentinuzzi, \{Veronica S.\} and Kolker, \{Daniel E.\} and Vitaterna, \{Martha Hotz\} and Kazuhiro Shimomura and Andrew Whiteley and Sharon Low-Zeddies and Turek, \{Fred W.\} and Ferrari, \{Elenice A.M.\} and Richard Paylor and Takahashi, \{Joseph S.\}",

year = "1998",

doi = "10.1101/lm.5.4.391",

language = "English",

volume = "5",

pages = "391--403",

journal = "Learning and Memory",

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Valentinuzzi, VS, Kolker, DE, Vitaterna, MH, Shimomura, K, Whiteley, A, Low-Zeddies, S, Turek, FW, Ferrari, EAM, Paylor, R & Takahashi, JS 1998, 'Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F₂ mice', Learning and Memory, vol. 5, no. 4-5, pp. 391-403. https://doi.org/10.1101/lm.5.4.391

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T1 - Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F2 mice

AU - Valentinuzzi, Veronica S.

AU - Kolker, Daniel E.

AU - Vitaterna, Martha Hotz

AU - Shimomura, Kazuhiro

AU - Whiteley, Andrew

AU - Low-Zeddies, Sharon

AU - Turek, Fred W.

AU - Ferrari, Elenice A.M.

AU - Paylor, Richard

AU - Takahashi, Joseph S.

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N2 - The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test.

AB - The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test.

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Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F₂ mice

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