Biochemical properties of macrophage fractions and their relation to the mechanism of Superoxide production

Michael S. Diamond; Andrij Holian

doi:10.1016/0014-5793(86)80290-3

Biochemical properties of macrophage fractions and their relation to the mechanism of Superoxide production

Michael S. Diamond
, Andrij Holian

University of Pennsylvania

Research output: Contribution to journal › Article › peer-review

Abstract

Guinea pig alveolar macrophages are separable by density gradient centrifugation into three subpopulations whose capacity for biological activity (e.g. O₂^-production and chemotaxis) varies directly with buoyant density [(1983) J. Reticuloendothel. Soc. 33, 157-164]. This study demonstrates that the activity per cell of various other enzymes remains constant among the subpopulations. When normalized for cell volume, enzyme activity diminishes with decreasing buoyant density. Intracellular calcium mobilization, linked to formyl peptide and concanavalin A-stimulated O₂^- production, similarly diminishes. Formyl peptide receptor distribution and affinity remain constant. Decreased responsiveness of lower density cells is probably due to lower concentration of enzyme(s) involved in the transduction of signal distal to ligand recognition (or binding).

Original language	English
Pages (from-to)	21-26
Number of pages	6
Journal	FEBS Letters
Volume	197
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(86)80290-3
State	Published - Mar 3 1986

Funding

This work was supportedb y grants HL-26266 and HL-28758f rom the National Heart, Lung and Blood Institute.

Keywords

Macrophage Percoll Formyl peptide Peptide receptor Ca

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10.1016/0014-5793(86)80290-3

Cite this

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title = "Biochemical properties of macrophage fractions and their relation to the mechanism of Superoxide production",

abstract = "Guinea pig alveolar macrophages are separable by density gradient centrifugation into three subpopulations whose capacity for biological activity (e.g. O2-production and chemotaxis) varies directly with buoyant density [(1983) J. Reticuloendothel. Soc. 33, 157-164]. This study demonstrates that the activity per cell of various other enzymes remains constant among the subpopulations. When normalized for cell volume, enzyme activity diminishes with decreasing buoyant density. Intracellular calcium mobilization, linked to formyl peptide and concanavalin A-stimulated O2- production, similarly diminishes. Formyl peptide receptor distribution and affinity remain constant. Decreased responsiveness of lower density cells is probably due to lower concentration of enzyme(s) involved in the transduction of signal distal to ligand recognition (or binding).",

keywords = "Macrophage Percoll Formyl peptide Peptide receptor Ca",

author = "Diamond, \{Michael S.\} and Andrij Holian",

note = "Funding Information: This work was supportedb y grants HL-26266 and HL-28758f rom the National Heart, Lung and Blood Institute.",

year = "1986",

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doi = "10.1016/0014-5793(86)80290-3",

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T1 - Biochemical properties of macrophage fractions and their relation to the mechanism of Superoxide production

AU - Diamond, Michael S.

AU - Holian, Andrij

N1 - Funding Information: This work was supportedb y grants HL-26266 and HL-28758f rom the National Heart, Lung and Blood Institute.

PY - 1986/3/3

Y1 - 1986/3/3

N2 - Guinea pig alveolar macrophages are separable by density gradient centrifugation into three subpopulations whose capacity for biological activity (e.g. O2-production and chemotaxis) varies directly with buoyant density [(1983) J. Reticuloendothel. Soc. 33, 157-164]. This study demonstrates that the activity per cell of various other enzymes remains constant among the subpopulations. When normalized for cell volume, enzyme activity diminishes with decreasing buoyant density. Intracellular calcium mobilization, linked to formyl peptide and concanavalin A-stimulated O2- production, similarly diminishes. Formyl peptide receptor distribution and affinity remain constant. Decreased responsiveness of lower density cells is probably due to lower concentration of enzyme(s) involved in the transduction of signal distal to ligand recognition (or binding).

AB - Guinea pig alveolar macrophages are separable by density gradient centrifugation into three subpopulations whose capacity for biological activity (e.g. O2-production and chemotaxis) varies directly with buoyant density [(1983) J. Reticuloendothel. Soc. 33, 157-164]. This study demonstrates that the activity per cell of various other enzymes remains constant among the subpopulations. When normalized for cell volume, enzyme activity diminishes with decreasing buoyant density. Intracellular calcium mobilization, linked to formyl peptide and concanavalin A-stimulated O2- production, similarly diminishes. Formyl peptide receptor distribution and affinity remain constant. Decreased responsiveness of lower density cells is probably due to lower concentration of enzyme(s) involved in the transduction of signal distal to ligand recognition (or binding).

KW - Macrophage Percoll Formyl peptide Peptide receptor Ca

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DO - 10.1016/0014-5793(86)80290-3

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ER -

Biochemical properties of macrophage fractions and their relation to the mechanism of Superoxide production

Abstract

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Keywords

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