A paired whole-lake experiment was conducted on two remote, atmospherically driven lakes in the Experimental Lakes Area (ELA) to examine the stresses of trophic condition on air-water exchange and settling fluxes of poly-chlorinated biphenyls (PCBs). Lake 227 (L227) and Lake 110 (L110) are similar in volume, surface area, and watershed area but differ significantly in trophic status. The two lakes have similar access to atmospheric PCBs but eutrophic L227 may exhibit enhanced air to water exchange due to greater biotic uptake of dissolved PCBs. Settling fluxes of PCB were significantly greater in eutrophic L227 (40 ng m-2 d-1 in 1993; 29 ng m-2 d-1 in 1994) than in oligotrophic L110 (22 ng m-2 d-1 in 1993 and 17 ng m-2 d-1 in 1994). Dissolved ΣPCB concentrations were not significantly different in the two lakes (~0.3 ng liter-1). Greater ΣPCB settling fluxes in L227 vs. L110 coupled with similar dissolved concentrations after June supports the hypothesis that air-water exchange supports the water column PCB concentrations. Surprisingly, ΣPCB fugacity gradients in both lakes indicated that net volatilization dominated during the entire ice-free period, requiting another source. A ΣPCB mass budget in the epilimnion of each lake over the stratified period showed that the major PCB losses were due to settling and volatilization. ΣPCB settling losses were 2.4 times greater in L227 than in L110 due to higher settling particle fluxes. The mass budgets suggest that the major PCB input to these remote lakes is from the watershed. Our results indicate the intimate environmental linkage between atmospheric, land, and aquatic PCB reservoirs.