Caenorhabditis elegans DLC-1 associates with ribonucleoprotein complexes to promote mRNA regulation

Nicholas J. Day; Mary Ellenbecker; Ekaterina Voronina

doi:10.1002/1873-3468.13259

Caenorhabditis elegans DLC-1 associates with ribonucleoprotein complexes to promote mRNA regulation

Nicholas J. Day
, Mary Ellenbecker
, Ekaterina Voronina

Division of Biological and Biomedical Sciences

University of Montana

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previously identified as a cofactor of two unrelated RNA-binding proteins that act in the Caenorhabditis elegans germline. Among the 2732 potential DLC-1 targets, most are germline mRNAs associated with oogenesis. Removal of DLC-1 affects expression of its targets expressed in the oocytes, meg-1 and meg-3. We propose that DLC-1 acts as a cofactor for multiple ribonucleoprotein complexes, including the ones regulating gene expression during oogenesis.

Original language	English
Pages (from-to)	3683-3695
Number of pages	13
Journal	FEBS Letters
Volume	592
Issue number	22
DOIs	https://doi.org/10.1002/1873-3468.13259
State	Published - Nov 2018

Funding

We thank Voronina lab members, Jennifer Wang, Tiago Antao, Micah Gearhart, Tim Schedl, Geraldine Seydoux, and two anonymous reviewers for experimental suggestions, provision of reagents, bioinformatics support, or comments on the manuscript. Some nematode strains were provided by the Caenorhabditis Genetics Center (P40OD010440). Confocal microscopy was facilitated by NIH awards S10OD021806 and P20GM103546. This work was supported by NIH R01GM109053 grant to EV, UM Research Award to ME, and UM ASUM award to ND. Funding sources had no involvement in study design and execution or writing the report. We thank Voronina lab members, Jennifer Wang, Tiago Antao, Micah Gearhart, Tim Schedl, Geraldine Seydoux, and two anonymous reviewers for experimental suggestions, provision of reagents, bioinformat-ics support, or comments on the manuscript. Some nematode strains were provided by the Caenorhabditis Genetics Center (P40OD010440). Confocal microscopy was facilitated by NIH awards S10OD021806 and P20GM103546. This work was supported by NIH R01GM109053 grant to EV, UM Research Award to ME, and UM ASUM award to ND. Funding sources had no involvement in study design and execution or writing the report.

Funder number
P40OD010440
P20GM103546, S10OD021806
R01GM109053

Keywords

LC8
RNA-binding protein
germline
oogenesis

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10.1002/1873-3468.13259

Cite this

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abstract = "Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previously identified as a cofactor of two unrelated RNA-binding proteins that act in the Caenorhabditis elegans germline. Among the 2732 potential DLC-1 targets, most are germline mRNAs associated with oogenesis. Removal of DLC-1 affects expression of its targets expressed in the oocytes, meg-1 and meg-3. We propose that DLC-1 acts as a cofactor for multiple ribonucleoprotein complexes, including the ones regulating gene expression during oogenesis.",

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AB - Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previously identified as a cofactor of two unrelated RNA-binding proteins that act in the Caenorhabditis elegans germline. Among the 2732 potential DLC-1 targets, most are germline mRNAs associated with oogenesis. Removal of DLC-1 affects expression of its targets expressed in the oocytes, meg-1 and meg-3. We propose that DLC-1 acts as a cofactor for multiple ribonucleoprotein complexes, including the ones regulating gene expression during oogenesis.

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Caenorhabditis elegans DLC-1 associates with ribonucleoprotein complexes to promote mRNA regulation

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Keywords

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