Cascading influence of inorganic nitrogen sources on DOM production, composition, lability and microbial community structure in the open ocean

S. J. Goldberg; C. E. Nelson; D. A. Viviani; C. N. Shulse; M. J. Church

doi:10.1111/1462-2920.13825

Cascading influence of inorganic nitrogen sources on DOM production, composition, lability and microbial community structure in the open ocean

S. J. Goldberg
, C. E. Nelson
, D. A. Viviani
, C. N. Shulse
, M. J. Church

Flathead Lake Biological Station

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Nitrogen frequently limits oceanic photosynthesis and the availability of inorganic nitrogen sources in the surface oceans is shifting with global change. We evaluated the potential for abrupt increases in inorganic N sources to induce cascading effects on dissolved organic matter (DOM) and microbial communities in the surface ocean. We collected water from 5 m depth in the central North Pacific and amended duplicate 20 liter polycarbonate carboys with nitrate or ammonium, tracking planktonic carbon fixation, DOM production, DOM composition and microbial community structure responses over 1 week relative to controls. Both nitrogen sources stimulated bulk phytoplankton, bacterial and DOM production and enriched Synechococcus and Flavobacteriaceae; ammonium enriched for oligotrophic Actinobacteria OM1 and Gammaproteobacteria KI89A clades while nitrate enriched Gammaproteobacteria SAR86, SAR92 and OM60 clades. DOM resulting from both N enrichments was more labile and stimulated growth of copiotrophic Gammaproteobacteria (Alteromonadaceae and Oceanospirillaceae) and Alphaproteobacteria (Rhodobacteraceae and Hyphomonadaceae) in weeklong dark incubations relative to controls. Our study illustrates how nitrogen pulses may have direct and cascading effects on DOM composition and microbial community dynamics in the open ocean.

Original language	English
Pages (from-to)	3450-3464
Number of pages	15
Journal	Environmental Microbiology
Volume	19
Issue number	9
DOIs	https://doi.org/10.1111/1462-2920.13825
State	Published - Sep 2017

Funding

We thank Susan Curless and Alexa Nelson for analyses of inorganic nutrients, and acknowledge the efforts of the Hawai’i Ocean Time-series staff for their support of this project. We thank Amy Eggers, director of the Evolutionary Genomics Core Facility, Hawai‘i Institute of Marine Biology, University of Hawai‘i at Maµnoa for DNA sequencing. The authors declare no conflict of interest. This work was supported by grants from the National Science Foundation (NSF) (OCE-1241263 and OCE-1260164 to MJC, OCE-1538393 to CEN), with additional support from the Simons Collaboration on Ocean Processes and Ecology (SCOPE to MJC) and the NSF Center for Microbial Oceanography: Research and Education (C-MORE, NSF EF-0424599). This paper is funded in part by a grant/cooperative agreement from the National Oceanic and Atmospheric Administration, Project A/AS-1, which is sponsored by the University of Hawaii Sea Grant College Program, SOEST, under Institutional Grant No. NA14OAR4170071 from NOAA Office of Sea Grant, Department of Commerce. The views expressed herein are those of the author(s) and do not necessarily reflect the views of NOAA or any of its subagencies. This article has been assigned the Hawaii Sea Grant publication number UNIHI-SEAGRANT-JC-15-21 and SOEST publication number 10061. The authors declare no conflict of interest.

Funders	Funder number
EF-0424599, OCE-1538393, OCE-1260164, 1538393, OCE-1241263
National Oceanic and Atmospheric Administration
NA14OAR4170071

Access to Document

10.1111/1462-2920.13825

Cite this

@article{7d4fbe9622114151920b8652220a98ea,

title = "Cascading influence of inorganic nitrogen sources on DOM production, composition, lability and microbial community structure in the open ocean",

abstract = "Nitrogen frequently limits oceanic photosynthesis and the availability of inorganic nitrogen sources in the surface oceans is shifting with global change. We evaluated the potential for abrupt increases in inorganic N sources to induce cascading effects on dissolved organic matter (DOM) and microbial communities in the surface ocean. We collected water from 5 m depth in the central North Pacific and amended duplicate 20 liter polycarbonate carboys with nitrate or ammonium, tracking planktonic carbon fixation, DOM production, DOM composition and microbial community structure responses over 1 week relative to controls. Both nitrogen sources stimulated bulk phytoplankton, bacterial and DOM production and enriched Synechococcus and Flavobacteriaceae; ammonium enriched for oligotrophic Actinobacteria OM1 and Gammaproteobacteria KI89A clades while nitrate enriched Gammaproteobacteria SAR86, SAR92 and OM60 clades. DOM resulting from both N enrichments was more labile and stimulated growth of copiotrophic Gammaproteobacteria (Alteromonadaceae and Oceanospirillaceae) and Alphaproteobacteria (Rhodobacteraceae and Hyphomonadaceae) in weeklong dark incubations relative to controls. Our study illustrates how nitrogen pulses may have direct and cascading effects on DOM composition and microbial community dynamics in the open ocean.",

author = "Goldberg, \{S. J.\} and Nelson, \{C. E.\} and Viviani, \{D. A.\} and Shulse, \{C. N.\} and Church, \{M. J.\}",

note = "Publisher Copyright: {\textcopyright} 2017 Society for Applied Microbiology and John Wiley \& Sons Ltd",

year = "2017",

month = sep,

doi = "10.1111/1462-2920.13825",

language = "English",

volume = "19",

pages = "3450--3464",

journal = "Environmental Microbiology",

issn = "1462-2912",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "9",

}

TY - JOUR

T1 - Cascading influence of inorganic nitrogen sources on DOM production, composition, lability and microbial community structure in the open ocean

AU - Goldberg, S. J.

AU - Nelson, C. E.

AU - Viviani, D. A.

AU - Shulse, C. N.

AU - Church, M. J.

PY - 2017/9

Y1 - 2017/9

N2 - Nitrogen frequently limits oceanic photosynthesis and the availability of inorganic nitrogen sources in the surface oceans is shifting with global change. We evaluated the potential for abrupt increases in inorganic N sources to induce cascading effects on dissolved organic matter (DOM) and microbial communities in the surface ocean. We collected water from 5 m depth in the central North Pacific and amended duplicate 20 liter polycarbonate carboys with nitrate or ammonium, tracking planktonic carbon fixation, DOM production, DOM composition and microbial community structure responses over 1 week relative to controls. Both nitrogen sources stimulated bulk phytoplankton, bacterial and DOM production and enriched Synechococcus and Flavobacteriaceae; ammonium enriched for oligotrophic Actinobacteria OM1 and Gammaproteobacteria KI89A clades while nitrate enriched Gammaproteobacteria SAR86, SAR92 and OM60 clades. DOM resulting from both N enrichments was more labile and stimulated growth of copiotrophic Gammaproteobacteria (Alteromonadaceae and Oceanospirillaceae) and Alphaproteobacteria (Rhodobacteraceae and Hyphomonadaceae) in weeklong dark incubations relative to controls. Our study illustrates how nitrogen pulses may have direct and cascading effects on DOM composition and microbial community dynamics in the open ocean.

AB - Nitrogen frequently limits oceanic photosynthesis and the availability of inorganic nitrogen sources in the surface oceans is shifting with global change. We evaluated the potential for abrupt increases in inorganic N sources to induce cascading effects on dissolved organic matter (DOM) and microbial communities in the surface ocean. We collected water from 5 m depth in the central North Pacific and amended duplicate 20 liter polycarbonate carboys with nitrate or ammonium, tracking planktonic carbon fixation, DOM production, DOM composition and microbial community structure responses over 1 week relative to controls. Both nitrogen sources stimulated bulk phytoplankton, bacterial and DOM production and enriched Synechococcus and Flavobacteriaceae; ammonium enriched for oligotrophic Actinobacteria OM1 and Gammaproteobacteria KI89A clades while nitrate enriched Gammaproteobacteria SAR86, SAR92 and OM60 clades. DOM resulting from both N enrichments was more labile and stimulated growth of copiotrophic Gammaproteobacteria (Alteromonadaceae and Oceanospirillaceae) and Alphaproteobacteria (Rhodobacteraceae and Hyphomonadaceae) in weeklong dark incubations relative to controls. Our study illustrates how nitrogen pulses may have direct and cascading effects on DOM composition and microbial community dynamics in the open ocean.

UR - https://www.scopus.com/pages/publications/85029365576

U2 - 10.1111/1462-2920.13825

DO - 10.1111/1462-2920.13825

M3 - Article

C2 - 28618153

AN - SCOPUS:85029365576

SN - 1462-2912

VL - 19

SP - 3450

EP - 3464

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 9

ER -

Cascading influence of inorganic nitrogen sources on DOM production, composition, lability and microbial community structure in the open ocean

Abstract

Funding

Access to Document

Other files and links

Fingerprint

Cite this