Cav3.2 T-type calcium channels control acute itch in mice

Vinicius M. Gadotti, Joanna M. Kreitinger, Nicholas B. Wageling, Dylan Budke, Philippe Diaz, Gerald W. Zamponi

Research output: Contribution to journalArticlepeer-review

Abstract

Cav3.2 T-type calcium channels are important mediators of nociceptive signaling, but their roles in the transmission of itch remains poorly understood. Here we report a key involvement of these channels as key modulators of itch/pruritus-related behavior. We compared scratching behavior responses between wild type and Cav3.2 null mice in models of histamine- or chloroquine-induced itch. We also evaluated the effect of the T-type calcium channel blocker DX332 in male and female wild-type mice injected with either histamine or chloroquine. Cav3.2 null mice exhibited decreased scratching responses during both histamine- and chloroquine-induced acute itch. DX332 co-injected with the pruritogens inhibited scratching responses of male and female mice treated with either histamine or chloroquine. Altogether, our data provide strong evidence that Cav3.2 T-type channels exert an important role in modulating histamine-dependent and -independent itch transmission in the primary sensory afferent pathway, and highlight these channels as potential pharmacological targets to treat pruritus.

Original languageEnglish
Article number119
JournalMolecular Brain
Volume13
Issue number1
DOIs
StatePublished - Sep 1 2020

Keywords

  • Cav3.2 T-type channel
  • Chloroquine
  • Histamine
  • Itch
  • Pruritus

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