Characterization of Drosophila octopamine receptor neuronal expression using MiMIC-converted Gal4 lines

Hannah M. McKinney, Lewis M. Sherer, Jessica L. Williams, Sarah J. Certel, R. Steven Stowers

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Octopamine, the invertebrate analog of norepinephrine, is known to modulate a large variety of behaviors in Drosophila including feeding initiation, locomotion, aggression, and courtship, among many others. Significantly less is known about the identity of the neurons that receive octopamine input and how they mediate octopamine-regulated behaviors. Here, we characterize adult neuronal expression of MiMIC-converted Trojan-Gal4 lines for each of the five Drosophila octopamine receptors. Broad neuronal expression was observed for all five octopamine receptors, yet distinct differences among them were also apparent. Use of immunostaining for the octopamine neurotransmitter synthesis enzyme Tdc2, along with a novel genome-edited conditional Tdc2-LexA driver, revealed all five octopamine receptors express in Tdc2/octopamine neurons to varying degrees. This suggests autoreception may be an important circuit mechanism by which octopamine modulates behavior.

Original languageEnglish
Pages (from-to)2174-2194
Number of pages21
JournalJournal of Comparative Neurology
Volume528
Issue number13
DOIs
StatePublished - Sep 1 2020

Keywords

  • Drosophila
  • RRID:AB_221568
  • RRID:AB_2340686
  • RRID:AB_2340850
  • RRID:AB_2536611
  • RRID:AB_2633280
  • RRID:AB_2814891
  • RRID:BDSC_27392
  • RRID:BDSC_42119
  • RRID:BDSC_43050
  • RRID:BDSC_57940
  • RRID:BDSC_59133
  • RRID:BDSC_60312
  • RRID:BDSC_60313
  • RRID:BDSC_67636
  • RRID:BDSC_68264
  • autoreception
  • octopamine
  • octopamine receptor

Fingerprint

Dive into the research topics of 'Characterization of Drosophila octopamine receptor neuronal expression using MiMIC-converted Gal4 lines'. Together they form a unique fingerprint.

Cite this