Characterization of reversible associations by sedimentation velocity with ultrascan

Borries Demeler, Emre Brookes, Renjing Wang, Virgil Schirf, Chongwoo A. Kim

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


We compare here the utility of sedimentation velocity (SV) to sedimentation equilibrium (SE) analysis for the characterization of reversible systems. Genetic algorithm optimization in UltraScan is used to optimize the model and to obtain solution properties of all components present in the system. We apply our method to synthetic and experimental data, and suggest limits for the accessible kinetic range. We conclude that equilibrium constants obtained from SV and SE analysis are equivalent, but that SV experiments provide better confidence for the Kd, can better account for the presence of contaminants and provide additional information including rate constants and shape parameters. (Graph Presented)

Original languageEnglish
Pages (from-to)775-782
Number of pages8
JournalMacromolecular Bioscience
Issue number7
StatePublished - Jul 7 2010


  • Analytical ultracentrifugation
  • Equilibrium complexes and rate constants
  • Monomers
  • Polycomb repression complex 1
  • RING1B
  • Reversible associations
  • Sedimentation velocity
  • Structure
  • Ultrascan


Dive into the research topics of 'Characterization of reversible associations by sedimentation velocity with ultrascan'. Together they form a unique fingerprint.

Cite this