@inbook{25f78a19a2664227ae8c42d990f2536f,
title = "Characterization of RVFV Nucleocapsid Protein Binding Sites on RNA by iCLIP-seq",
abstract = "The nucleocapsid protein (N) in Rift Valley fever virus is an RNA-binding protein that functions in viral transcription, replication, and packaging. In this chapter, the method for studying protein-RNA interactions in context of viral infection using individual nucleotide resolution, cross-linking, immunoprecipitation, and sequencing (iCLIP-seq) is explained. The method is useful for identifying the interactions between both host and viral RNAs with N and can identify RNA motifs that interact with the protein of interest.",
keywords = "iCLIP, Nucleocapsid protein, Rift Valley fever virus, RNA",
author = "Miyuki Hayashi and Lodmell, {J. Stephen}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3926-9_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "319--334",
booktitle = "Methods in Molecular Biology",
}