Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

Michael F. Minnick; Joan C. Strange; Kurt F. Williams

doi:10.1016/0378-1119(94)90622-X

Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

Michael F. Minnick, Joan C. Strange, Kurt F. Williams

Biological Sciences

University of Montana

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The 16S-23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNA^Ile) and alanine-tRNA (tRNA^Ala). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S-23S spacer region, with approx. 300 flanking nt. Genes encoding tRNA^Ile and tRNA^Ala have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.

Original language	English
Pages (from-to)	149-150
Number of pages	2
Journal	Gene
Volume	143
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90622-X
State	Published - May 27 1994

Keywords

Oroya fever
Rickettsiales
diagnostic tool
hemotrophic pathogenic bacteria
nucleotide sequence
ribosomal RNA operon
tRNA
tRNA

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10.1016/0378-1119(94)90622-X

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@article{a9534abad657443d81ff1c760e2daba1,

title = "Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis",

abstract = "The 16S-23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNAIle) and alanine-tRNA (tRNAAla). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S-23S spacer region, with approx. 300 flanking nt. Genes encoding tRNAIle and tRNAAla have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.",

keywords = "Oroya fever, Rickettsiales, diagnostic tool, hemotrophic pathogenic bacteria, nucleotide sequence, ribosomal RNA operon, tRNA, tRNA",

author = "Minnick, {Michael F.} and Strange, {Joan C.} and Williams, {Kurt F.}",

note = "Funding Information: This work was supported by funds from the University of Montana URCAC and MONTS (NSF-EPSCoR) program (#RI1 8912934). Synthetic oligodeoxynucleotides were provided by the University of Montana Murdoch Molecular Biology Facility.",

year = "1994",

month = may,

day = "27",

doi = "10.1016/0378-1119(94)90622-X",

language = "English",

volume = "143",

pages = "149--150",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

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T1 - Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

AU - Minnick, Michael F.

AU - Strange, Joan C.

AU - Williams, Kurt F.

N1 - Funding Information: This work was supported by funds from the University of Montana URCAC and MONTS (NSF-EPSCoR) program (#RI1 8912934). Synthetic oligodeoxynucleotides were provided by the University of Montana Murdoch Molecular Biology Facility.

PY - 1994/5/27

Y1 - 1994/5/27

N2 - The 16S-23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNAIle) and alanine-tRNA (tRNAAla). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S-23S spacer region, with approx. 300 flanking nt. Genes encoding tRNAIle and tRNAAla have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.

AB - The 16S-23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNAIle) and alanine-tRNA (tRNAAla). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S-23S spacer region, with approx. 300 flanking nt. Genes encoding tRNAIle and tRNAAla have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.

KW - Oroya fever

KW - Rickettsiales

KW - diagnostic tool

KW - hemotrophic pathogenic bacteria

KW - nucleotide sequence

KW - ribosomal RNA operon

KW - tRNA

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DO - 10.1016/0378-1119(94)90622-X

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AN - SCOPUS:0028352269

SN - 0378-1119

VL - 143

SP - 149

EP - 150

JO - Gene

JF - Gene

IS - 1

ER -

Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

Abstract

Keywords

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