Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

Michael F. Minnick, Joan C. Strange, Kurt F. Williams

Research output: Contribution to journalArticlepeer-review

Abstract

The 16S-23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNAIle) and alanine-tRNA (tRNAAla). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S-23S spacer region, with approx. 300 flanking nt. Genes encoding tRNAIle and tRNAAla have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.

Original languageEnglish
Pages (from-to)149-150
Number of pages2
JournalGene
Volume143
Issue number1
DOIs
StatePublished - May 27 1994

Keywords

  • diagnostic tool
  • hemotrophic pathogenic bacteria
  • nucleotide sequence
  • Oroya fever
  • ribosomal RNA operon
  • Rickettsiales
  • tRNA
  • tRNA

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