Characterization of the denatured state

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

5 Scopus citations

Abstract

In this chapter, the current state of knowledge of protein denatured states is reviewed. Flory's random coil model and recent theory and simulation approaches to protein denatured states are outlined. Nuclear magnetic resonance and fluorescence methods and the insights they have provided into residual secondary structure and transient tertiary interactions in the denatured state are discussed. Recent techniques that use experimental restraints to produce representative ensembles for the denatured state are presented. The thermodynamic and kinetic properties of the denatured state and their implications for folding and stability are considered. Finally, the prospects for future studies on protein denatured states are briefly summarized.

Original languageEnglish
Title of host publicationComprehensive Biophysics
PublisherElsevier Inc.
Pages72-114
Number of pages43
Volume3
ISBN (Print)9780080957180
DOIs
StatePublished - 2012

Keywords

  • Denatured state electrostatics
  • Fluorescence resonance energy transfer
  • Hydrogen exchange superprotection
  • Loop formation kinetics
  • Loop formation thermodynamics
  • Molecular dynamics
  • Paramagnetic relaxation enhancement
  • Protein denatured states
  • Radius of gyration
  • Random coil
  • Residual dipolar coupling
  • Residual structure
  • Scaling exponents
  • Single molecule fluorescence

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