Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-β-lipotropin precursor

S. Nakanishi; A. Inoue; T. Kita; S. Numa; A. C. Chang; S. N. Cohen; J. Nunberg; R. T. Schimke

doi:10.1073/pnas.75.12.6021

Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-β-lipotropin precursor

S. Nakanishi, A. Inoue, T. Kita, S. Numa, A. C. Chang, S. N. Cohen, J. Nunberg, R. T. Schimke

Kyoto University

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

mRNA that encodes the common peptide precursor for the hormones corticotropin and β-lipotropin was purified from the neurointermediate lobe of bovine pituitaries, and double-stranded cDNA species synthesized from this template were cloned in Escherichia coli χ 1776 by inserting them into the Pst I endonuclease cleavage site of the pBR322 plasmid using poly(dG)poly(dC) homopolymeric extensions. Certain of the cloned cDNA inserts contain nucleotides corresponding to the complete amino acid sequence of bovine corticotropin and a coding sequence that corresponds to at least the first portion of bovine β-lipotropin. The nucleotide sequences coding for corticotropin and β-lipotropin are separated on the cDNA by a 6-base-pair sequence encoding lysine and arginine, indicating that the carboxyl terminus of corticotropin is connected on the precursor peptide with the amino terminus of β-lipotropin by these two amino acids. In addition, the cloned cDNA insert is characterized by an unusually high C+G nucleotide base content as well as by a number of DNA sequence duplications.

Original language	English
Pages (from-to)	6021-6025
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	75
Issue number	12
DOIs	https://doi.org/10.1073/pnas.75.12.6021
State	Published - 1978

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Nakanishi, S., Inoue, A., Kita, T., Numa, S., Chang, A. C., Cohen, S. N., Nunberg, J., & Schimke, R. T. (1978). Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-β-lipotropin precursor. Proceedings of the National Academy of Sciences of the United States of America, 75(12), 6021-6025. https://doi.org/10.1073/pnas.75.12.6021

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title = "Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-β-lipotropin precursor",

abstract = "mRNA that encodes the common peptide precursor for the hormones corticotropin and β-lipotropin was purified from the neurointermediate lobe of bovine pituitaries, and double-stranded cDNA species synthesized from this template were cloned in Escherichia coli χ 1776 by inserting them into the Pst I endonuclease cleavage site of the pBR322 plasmid using poly(dG)poly(dC) homopolymeric extensions. Certain of the cloned cDNA inserts contain nucleotides corresponding to the complete amino acid sequence of bovine corticotropin and a coding sequence that corresponds to at least the first portion of bovine β-lipotropin. The nucleotide sequences coding for corticotropin and β-lipotropin are separated on the cDNA by a 6-base-pair sequence encoding lysine and arginine, indicating that the carboxyl terminus of corticotropin is connected on the precursor peptide with the amino terminus of β-lipotropin by these two amino acids. In addition, the cloned cDNA insert is characterized by an unusually high C+G nucleotide base content as well as by a number of DNA sequence duplications.",

author = "S. Nakanishi and A. Inoue and T. Kita and S. Numa and Chang, {A. C.} and Cohen, {S. N.} and J. Nunberg and Schimke, {R. T.}",

year = "1978",

doi = "10.1073/pnas.75.12.6021",

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Nakanishi, S, Inoue, A, Kita, T, Numa, S, Chang, AC, Cohen, SN, Nunberg, J & Schimke, RT 1978, 'Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-β-lipotropin precursor', Proceedings of the National Academy of Sciences of the United States of America, vol. 75, no. 12, pp. 6021-6025. https://doi.org/10.1073/pnas.75.12.6021

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T1 - Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-β-lipotropin precursor

AU - Nakanishi, S.

AU - Inoue, A.

AU - Kita, T.

AU - Numa, S.

AU - Chang, A. C.

AU - Cohen, S. N.

AU - Nunberg, J.

AU - Schimke, R. T.

PY - 1978

Y1 - 1978

N2 - mRNA that encodes the common peptide precursor for the hormones corticotropin and β-lipotropin was purified from the neurointermediate lobe of bovine pituitaries, and double-stranded cDNA species synthesized from this template were cloned in Escherichia coli χ 1776 by inserting them into the Pst I endonuclease cleavage site of the pBR322 plasmid using poly(dG)poly(dC) homopolymeric extensions. Certain of the cloned cDNA inserts contain nucleotides corresponding to the complete amino acid sequence of bovine corticotropin and a coding sequence that corresponds to at least the first portion of bovine β-lipotropin. The nucleotide sequences coding for corticotropin and β-lipotropin are separated on the cDNA by a 6-base-pair sequence encoding lysine and arginine, indicating that the carboxyl terminus of corticotropin is connected on the precursor peptide with the amino terminus of β-lipotropin by these two amino acids. In addition, the cloned cDNA insert is characterized by an unusually high C+G nucleotide base content as well as by a number of DNA sequence duplications.

AB - mRNA that encodes the common peptide precursor for the hormones corticotropin and β-lipotropin was purified from the neurointermediate lobe of bovine pituitaries, and double-stranded cDNA species synthesized from this template were cloned in Escherichia coli χ 1776 by inserting them into the Pst I endonuclease cleavage site of the pBR322 plasmid using poly(dG)poly(dC) homopolymeric extensions. Certain of the cloned cDNA inserts contain nucleotides corresponding to the complete amino acid sequence of bovine corticotropin and a coding sequence that corresponds to at least the first portion of bovine β-lipotropin. The nucleotide sequences coding for corticotropin and β-lipotropin are separated on the cDNA by a 6-base-pair sequence encoding lysine and arginine, indicating that the carboxyl terminus of corticotropin is connected on the precursor peptide with the amino terminus of β-lipotropin by these two amino acids. In addition, the cloned cDNA insert is characterized by an unusually high C+G nucleotide base content as well as by a number of DNA sequence duplications.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-β-lipotropin precursor

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