Control of net community production by microbial community respiration at Station ALOHA

Sandra Martínez-García; Robert R. Bidigare; Daniela A. del Valle; Laurie W. Juranek; David P. Nicholson; Donn A. Viviani; Samuel T. Wilson; Matthew J. Church

doi:10.1016/j.jmarsys.2018.03.007

Control of net community production by microbial community respiration at Station ALOHA

Sandra Martínez-García
, Robert R. Bidigare
, Daniela A. del Valle
, Laurie W. Juranek
, David P. Nicholson
, Donn A. Viviani
, Samuel T. Wilson
, Matthew J. Church

Flathead Lake Biological Station

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Net community production (NCP) constrains the amount of carbon that is available for storage within or export from, the photic zone of marine ecosystems. With the aim of studying the control exerted by microbial community respiration (MCR) and gross primary production (GPP) over NCP, short-term variability in rates of MCR, GPP and NCP were measured over a 13-day sampling period (August 23–September 4, 2012) at Station ALOHA. During this period, picoplankton abundances and concentrations of photosynthetic pigments demonstrated weak to moderate decreases coincident with the passage of a low salinity water mass through the sampling area. During the same period, rates of MCR, measured using the in vivo INT method, varied by 2.7-fold. Rates of primary production (PP) were estimated based on four different methodologies: Fast Repetition Rate Fluorometry (FRRF), ¹⁴C-bicarbonate assimilation, diel changes in mixed layer O₂ concentrations based on Seaglider measurements, and isotopic composition (¹⁷ΔO₂) of dissolved O₂. Remarkably, these different methods for measuring microbial metabolism were consistent, suggesting that collectively these different approaches provide an accurate constraint on ecosystem metabolism, despite the different time and space scales over which these measurements integrate. FRRF-derived estimates of PP, which were similar in magnitude to ¹⁴C-based-PP, were significantly correlated with variability in mixed layer MCR. None of the other independent measurements of PP demonstrated significant relationships to MCR. Day-to-day variability in MCR and bacterial growth efficiency (BGE), derived based on measurements of bacterial production (as estimated by ³H-leucine incorporation) and MCR, were related to changes in NCP estimated from O₂/Ar ratios (O₂/Ar-NCP), suggesting short-term variability in the efficiency with which microorganisms consume dissolved organic matter exerts direct control on rates of NCP in this ecosystem. Our results highlight internal consistency among the derived rates of ecosystem metabolism using a suite of different methodologies to estimate productivity and respiration. Moreover, we find that rates of MCR can be as variable as PP and that variability in MCR may regulate NCP in this ecosystem.

Original language	English
Pages (from-to)	28-35
Number of pages	8
Journal	Journal of Marine Systems
Volume	184
DOIs	https://doi.org/10.1016/j.jmarsys.2018.03.007
State	Published - Aug 2018

Funding

We thank the captain and crew of the R/V Kilo Moana for safe and efficient operations. We thank Ken Doggett and Sara Ferrón for their help with flow cytometry measurements and O 2 /Ar calculations, respectively. We acknowledge David M. Karl for support during this investigation and for comments on previous versions of this manuscript. Additional thanks to Beatriz Mouriño-Carballido and Sara Ferrón for comments on previous versions of this manuscript. We are grateful to two anonymous reviewers for their constructive comments which improved this manuscript. This research was supported by the National Science Foundation through the Center for Microbial Oceanography: Research and Education (C-MORE; EF04-24599 to D.M. Karl), the Gordon and Betty Moore Foundation (# 3794 to D.M. Karl) and the HOT program ( OCE-0926766 and OCE-1260164 to M.J. Church and D.M. Karl). We used data from the WHOTS mooring, which is supported by the National Oceanic and Atmospheric Administration (NOAA) and NSF grants. S. Martínez-García was funded by a C-MORE, and Fellowsea fellowships.

Funders	Funder number
Daniel K. Inouye Center for Microbial Oceanography: Research and Education
EF04-24599
National Oceanic and Atmospheric Administration
OCE-0926766, 3794, OCE-1260164

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Euphotic zone
Microbial community respiration
Net community production
Primary production
Station ALOHA

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10.1016/j.jmarsys.2018.03.007

Cite this

@article{0eda6f26a61b499f95de97ce954e73a6,

title = "Control of net community production by microbial community respiration at Station ALOHA",

abstract = "Net community production (NCP) constrains the amount of carbon that is available for storage within or export from, the photic zone of marine ecosystems. With the aim of studying the control exerted by microbial community respiration (MCR) and gross primary production (GPP) over NCP, short-term variability in rates of MCR, GPP and NCP were measured over a 13-day sampling period (August 23–September 4, 2012) at Station ALOHA. During this period, picoplankton abundances and concentrations of photosynthetic pigments demonstrated weak to moderate decreases coincident with the passage of a low salinity water mass through the sampling area. During the same period, rates of MCR, measured using the in vivo INT method, varied by 2.7-fold. Rates of primary production (PP) were estimated based on four different methodologies: Fast Repetition Rate Fluorometry (FRRF), 14C-bicarbonate assimilation, diel changes in mixed layer O2 concentrations based on Seaglider measurements, and isotopic composition (17ΔO2) of dissolved O2. Remarkably, these different methods for measuring microbial metabolism were consistent, suggesting that collectively these different approaches provide an accurate constraint on ecosystem metabolism, despite the different time and space scales over which these measurements integrate. FRRF-derived estimates of PP, which were similar in magnitude to 14C-based-PP, were significantly correlated with variability in mixed layer MCR. None of the other independent measurements of PP demonstrated significant relationships to MCR. Day-to-day variability in MCR and bacterial growth efficiency (BGE), derived based on measurements of bacterial production (as estimated by 3H-leucine incorporation) and MCR, were related to changes in NCP estimated from O2/Ar ratios (O2/Ar-NCP), suggesting short-term variability in the efficiency with which microorganisms consume dissolved organic matter exerts direct control on rates of NCP in this ecosystem. Our results highlight internal consistency among the derived rates of ecosystem metabolism using a suite of different methodologies to estimate productivity and respiration. Moreover, we find that rates of MCR can be as variable as PP and that variability in MCR may regulate NCP in this ecosystem.",

keywords = "Euphotic zone, Microbial community respiration, Net community production, Primary production, Station ALOHA",

author = "Sandra Mart{\'i}nez-Garc{\'i}a and Bidigare, \{Robert R.\} and \{del Valle\}, \{Daniela A.\} and Juranek, \{Laurie W.\} and Nicholson, \{David P.\} and Viviani, \{Donn A.\} and Wilson, \{Samuel T.\} and Church, \{Matthew J.\}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = aug,

doi = "10.1016/j.jmarsys.2018.03.007",

language = "English",

volume = "184",

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T1 - Control of net community production by microbial community respiration at Station ALOHA

AU - Martínez-García, Sandra

AU - Bidigare, Robert R.

AU - del Valle, Daniela A.

AU - Juranek, Laurie W.

AU - Nicholson, David P.

AU - Viviani, Donn A.

AU - Wilson, Samuel T.

AU - Church, Matthew J.

PY - 2018/8

Y1 - 2018/8

N2 - Net community production (NCP) constrains the amount of carbon that is available for storage within or export from, the photic zone of marine ecosystems. With the aim of studying the control exerted by microbial community respiration (MCR) and gross primary production (GPP) over NCP, short-term variability in rates of MCR, GPP and NCP were measured over a 13-day sampling period (August 23–September 4, 2012) at Station ALOHA. During this period, picoplankton abundances and concentrations of photosynthetic pigments demonstrated weak to moderate decreases coincident with the passage of a low salinity water mass through the sampling area. During the same period, rates of MCR, measured using the in vivo INT method, varied by 2.7-fold. Rates of primary production (PP) were estimated based on four different methodologies: Fast Repetition Rate Fluorometry (FRRF), 14C-bicarbonate assimilation, diel changes in mixed layer O2 concentrations based on Seaglider measurements, and isotopic composition (17ΔO2) of dissolved O2. Remarkably, these different methods for measuring microbial metabolism were consistent, suggesting that collectively these different approaches provide an accurate constraint on ecosystem metabolism, despite the different time and space scales over which these measurements integrate. FRRF-derived estimates of PP, which were similar in magnitude to 14C-based-PP, were significantly correlated with variability in mixed layer MCR. None of the other independent measurements of PP demonstrated significant relationships to MCR. Day-to-day variability in MCR and bacterial growth efficiency (BGE), derived based on measurements of bacterial production (as estimated by 3H-leucine incorporation) and MCR, were related to changes in NCP estimated from O2/Ar ratios (O2/Ar-NCP), suggesting short-term variability in the efficiency with which microorganisms consume dissolved organic matter exerts direct control on rates of NCP in this ecosystem. Our results highlight internal consistency among the derived rates of ecosystem metabolism using a suite of different methodologies to estimate productivity and respiration. Moreover, we find that rates of MCR can be as variable as PP and that variability in MCR may regulate NCP in this ecosystem.

AB - Net community production (NCP) constrains the amount of carbon that is available for storage within or export from, the photic zone of marine ecosystems. With the aim of studying the control exerted by microbial community respiration (MCR) and gross primary production (GPP) over NCP, short-term variability in rates of MCR, GPP and NCP were measured over a 13-day sampling period (August 23–September 4, 2012) at Station ALOHA. During this period, picoplankton abundances and concentrations of photosynthetic pigments demonstrated weak to moderate decreases coincident with the passage of a low salinity water mass through the sampling area. During the same period, rates of MCR, measured using the in vivo INT method, varied by 2.7-fold. Rates of primary production (PP) were estimated based on four different methodologies: Fast Repetition Rate Fluorometry (FRRF), 14C-bicarbonate assimilation, diel changes in mixed layer O2 concentrations based on Seaglider measurements, and isotopic composition (17ΔO2) of dissolved O2. Remarkably, these different methods for measuring microbial metabolism were consistent, suggesting that collectively these different approaches provide an accurate constraint on ecosystem metabolism, despite the different time and space scales over which these measurements integrate. FRRF-derived estimates of PP, which were similar in magnitude to 14C-based-PP, were significantly correlated with variability in mixed layer MCR. None of the other independent measurements of PP demonstrated significant relationships to MCR. Day-to-day variability in MCR and bacterial growth efficiency (BGE), derived based on measurements of bacterial production (as estimated by 3H-leucine incorporation) and MCR, were related to changes in NCP estimated from O2/Ar ratios (O2/Ar-NCP), suggesting short-term variability in the efficiency with which microorganisms consume dissolved organic matter exerts direct control on rates of NCP in this ecosystem. Our results highlight internal consistency among the derived rates of ecosystem metabolism using a suite of different methodologies to estimate productivity and respiration. Moreover, we find that rates of MCR can be as variable as PP and that variability in MCR may regulate NCP in this ecosystem.

KW - Euphotic zone

KW - Microbial community respiration

KW - Net community production

KW - Primary production

KW - Station ALOHA

UR - https://www.scopus.com/pages/publications/85046449103

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DO - 10.1016/j.jmarsys.2018.03.007

M3 - Article

AN - SCOPUS:85046449103

SN - 0924-7963

VL - 184

SP - 28

EP - 35

JO - Journal of Marine Systems

JF - Journal of Marine Systems

ER -

Control of net community production by microbial community respiration at Station ALOHA

Abstract

Funding

UN SDGs

Keywords

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