TY - JOUR
T1 - Coping with iron limitation
T2 - a metabolomic study of Synechocystis sp. PCC 6803
AU - Rivas-Ubach, Albert
AU - Poret-Peterson, Amisha T.
AU - Peñuelas, Josep
AU - Sardans, Jordi
AU - Pérez-Trujillo, Míriam
AU - Legido-Quigley, Cristina
AU - Oravec, Michal
AU - Urban, Otmar
AU - Elser, James J.
N1 - Publisher Copyright:
© 2018, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N2-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA:DNA, C, N, and P, and higher ratios of protein:RNA, C:N, C:P, and N:P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C:P and N:P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn, and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. In addition, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular, and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, may further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems.
AB - Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N2-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA:DNA, C, N, and P, and higher ratios of protein:RNA, C:N, C:P, and N:P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C:P and N:P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn, and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. In addition, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular, and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, may further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems.
KW - Cyanobacteria
KW - Ecological stoichiometry
KW - Iron limitation
KW - Metabolomics
KW - Metallomics
UR - http://www.scopus.com/inward/record.url?scp=85044352921&partnerID=8YFLogxK
U2 - 10.1007/s11738-018-2603-1
DO - 10.1007/s11738-018-2603-1
M3 - Article
AN - SCOPUS:85044352921
SN - 0137-5881
VL - 40
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 2
M1 - 28
ER -