Covalent modification of fused silica capillaries with quaternized polyamines to achieve robust and stable anodic electroosmotic flow

Jesse J. Stine, Christopher P. Palmer

Research output: Contribution to journalArticlepeer-review

Abstract

Synthetic chemistry originally developed for the manufacture of chemically stable silica polyamine composites was adapted for the modification of fused silica capillaries for application in CE. Polyethyleneimine (PEI) and polyallylamine (PAA) were covalently bonded to the interior surface of fused silica capillaries utilizing 3-chloropropyltrichlorosilane (CPTCS) or 3-glycidoxypropyl-trimethoxysilane (GPTMS) to anchor the polymers to the surface. The surface-bound polymers were subsequently quaternized using methyl iodide (MeI). The resulting modified capillaries were studied using CE, and were shown to provide reproducible, stable, and robust anodic EOF throughout the pH range of 2-10. Surface modifications utilizing CPTCS could be rinsed with up to 6 M HCL or 1 M NaOH without significant loss of surface modifier. The utility of the modified capillaries for the separation of simple anions and cations was demonstrated.

Original languageEnglish
Pages (from-to)446-456
Number of pages11
JournalJournal of Separation Science
Volume32
Issue number3
DOIs
StatePublished - Feb 2009

Keywords

  • Capillary modification
  • Polyamine

Fingerprint

Dive into the research topics of 'Covalent modification of fused silica capillaries with quaternized polyamines to achieve robust and stable anodic electroosmotic flow'. Together they form a unique fingerprint.

Cite this