Abstract
Several different crystal forms of Giα1 have been grown and analyzed. Crystals of native protein containing bound GTPγS belong to space group P3121 or P3221 with cell dimensions a, b = 80·6 Åand c = 106·3 Å and diffract to a resolution of 1·9 Å using synchrotron radiation. Crystals of native protein containing bound GDP belong to space group I4 with cell dimensions a, b = 121·3 Å, and c = 67·7 Å and diffract to 3·0Å. Data sets from crystals grown using mutant proteins have also been obtained and characterized.
Original language | English |
---|---|
Pages (from-to) | 630-634 |
Number of pages | 5 |
Journal | Journal of Molecular Biology |
Volume | 238 |
Issue number | 4 |
DOIs | |
State | Published - May 12 1994 |
Keywords
- G proteins
- GTP binding proteins
- Protein crystallization
- Signal transduction
- X-ray diffraction