Transboundary issues, like climate change, threaten the health of natural ecosystems. To address these problems, management is taking place at larger geographic scales and across political boundaries. Although landscapes are considered the suitable scale for conservation, limited research exists on how members of large landscape conservation organizations (LLCOs) define success. The Crown Managers Partnership (CMP) and the Southern Appalachian Man and the Biosphere Cooperative (SAMAB) are two LLCOs that support collaboration for the conservation of the Northern Rockies and the Southern Appalachian regions. We analyze the diverse interpretations of success among SAMAB and CMP participants during different stages of the organizations’ life cycle. Of particular importance is how members emphasize intangible successes such as relationships and the cascading effects LLCOs can have on other projects and organizations. This research provides insight into the distinct niche LLCOs fill in transboundary ecosystem management.