Design and evaluation of a novel fluorescent CB2 ligand as probe for receptor visualization in immune cells

Ravil R. Petrov, Maria E. Ferrini, Zeina Jaffar, Charles M. Thompson, Kevan Roberts, Philippe Diaz

Research output: Contribution to journalArticlepeer-review

Abstract

Cannabinoid CB2 receptor has emerged as a very promising target over the last decades. We have synthesized and evaluated a new fluorescent probe designated NMP6 based on 6-methoxyisatin scaffold, which exhibited selectivity and K i value at hCB2 of 387 nM. We have demonstrated its ability to be an effective probe for visualization of CB2 receptor binding using confocal microscopy and a flow cytometry probe for the analysis of CB2 protein expression. Furthermore, NMP6 was easily obtained in two chemical steps from commercially available building blocks.

Original languageEnglish
Pages (from-to)5859-5862
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume21
Issue number19
DOIs
StatePublished - Oct 1 2011

Keywords

  • CB2
  • Cannabinoid receptor 2
  • Fluorescent probe
  • GPCR

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