Detection of V-type nerve agent degradation products at electrodes modified by PPy/PQQ using CaCl2 as supporting electrolyte

Olga V. Shulga, Christopher Palmer

Research output: Contribution to journalArticlepeer-review

Abstract

Electrochemical detection without derivatization was used to detect thiol-containing degradation products of V-type nerve agents. Electropolymerization of pyrrole was used for entrapment of the biocatalyst PQQ to produce a sensor. Various parameters which affect the detection processes such as the type of the supporting electrolyte used during electrodeposition and the thickness of the polypyrrole film were examined and optimized. Electocatalytic oxidation of thiols by the PPy/PQQ electrode was strongly affected by the presence of Ca2+ cations during electrodeposition of the PPy/PQQ. Cyclic voltammetry, linear sweep voltammetry and amperometry have been used for electrode characterization. Amperometric detection of the V-type nerve agent thiol degradation products 2-(dimethylamino)ethanethiol (DMAET) and 2-(diethylamino)ethanethiol (DEAET) was performed at 0.38 V. Linear calibration plots were observed for these compounds. The detection limits of 4.5 and 3 μM were obtained for DMAET and DEAET respectively, with sensitivities of 1.18 and 1.37 nA μM-1 cm-2.

Original languageEnglish
Pages (from-to)1116-1123
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume385
Issue number6
DOIs
StatePublished - Jul 2006

Keywords

  • Amperometry
  • Chemical warfare agents
  • Electrodeposition
  • Polypyrrole
  • Pyrroloquinoline quinone
  • Thiols

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