TY - JOUR
T1 - Development and evaluation of a selective medium for Brucella suis
AU - Ferreira, Ana Cristina
AU - Almendra, Cláudia
AU - Cardoso, Regina
AU - Pereira, Marta Silva
AU - Beja-Pereira, Albano
AU - Luikart, Gordon
AU - Corrêa de Sá, Maria Inácia
N1 - Funding Information:
This work was financed by project Grants from FCT ( PTDC/CVT/69438/2006 and PTDC/CVT/104050/2008 ). CA was supported by a National Grant ( SFRH/BD30737/2006 ) Fundação para a Ciência e Tecnologia (FCT) and GL was partially supported by Grants from the US National Science Foundation (Grant DEB 074218 ), the Walton Family Foundation, CIBIO-UP , and Grants from the Portuguese Science Foundation ( PTDC/BIA-BDE/65625/2006 and PTDC/CVT/69438/2006 ).
PY - 2012/10
Y1 - 2012/10
N2 - A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.
AB - A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.
KW - Bacteriological diagnosis
KW - Brucella suis
KW - Selective medium
KW - Wild boar
UR - http://www.scopus.com/inward/record.url?scp=84863102696&partnerID=8YFLogxK
U2 - 10.1016/j.rvsc.2011.09.004
DO - 10.1016/j.rvsc.2011.09.004
M3 - Article
C2 - 21968103
AN - SCOPUS:84863102696
SN - 0034-5288
VL - 93
SP - 565
EP - 567
JO - Research in Veterinary Science
JF - Research in Veterinary Science
IS - 2
ER -