Development and evaluation of a selective medium for Brucella suis

Ana Cristina Ferreira; Cláudia Almendra; Regina Cardoso; Marta Silva Pereira; Albano Beja-Pereira; Gordon Luikart; Maria Inácia Corrêa de Sá

doi:10.1016/j.rvsc.2011.09.004

Development and evaluation of a selective medium for Brucella suis

Ana Cristina Ferreira
, Cláudia Almendra
, Regina Cardoso
, Marta Silva Pereira
, Albano Beja-Pereira
, Gordon Luikart
, Maria Inácia Corrêa de Sá

Flathead Lake Biological Station

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.

Original language	English
Pages (from-to)	565-567
Number of pages	3
Journal	Research in Veterinary Science
Volume	93
Issue number	2
DOIs	https://doi.org/10.1016/j.rvsc.2011.09.004
State	Published - Oct 2012

Funding

This work was financed by project Grants from FCT ( PTDC/CVT/69438/2006 and PTDC/CVT/104050/2008 ). CA was supported by a National Grant ( SFRH/BD30737/2006 ) Fundação para a Ciência e Tecnologia (FCT) and GL was partially supported by Grants from the US National Science Foundation (Grant DEB 074218 ), the Walton Family Foundation, CIBIO-UP , and Grants from the Portuguese Science Foundation ( PTDC/BIA-BDE/65625/2006 and PTDC/CVT/69438/2006 ).

Funder number
1067613, DEB 074218
PTDC/BIA-BDE/65625/2006

Keywords

Bacteriological diagnosis
Brucella suis
Selective medium
Wild boar

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10.1016/j.rvsc.2011.09.004

Cite this

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title = "Development and evaluation of a selective medium for Brucella suis",

abstract = "A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4\%) samples from 63 (6.9\%) animals resulted in a positive culture. LNIV-M detected 93.6\% and 62.6\% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1\% and 79.4\% of positive animals and 58.3\% and 59.7\% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.",

keywords = "Bacteriological diagnosis, Brucella suis, Selective medium, Wild boar",

author = "Ferreira, \{Ana Cristina\} and Cl{\'a}udia Almendra and Regina Cardoso and Pereira, \{Marta Silva\} and Albano Beja-Pereira and Gordon Luikart and \{Corr{\^e}a de S{\'a}\}, \{Maria In{\'a}cia\}",

note = "Funding Information: This work was financed by project Grants from FCT ( PTDC/CVT/69438/2006 and PTDC/CVT/104050/2008 ). CA was supported by a National Grant ( SFRH/BD30737/2006 ) Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (FCT) and GL was partially supported by Grants from the US National Science Foundation (Grant DEB 074218 ), the Walton Family Foundation, CIBIO-UP , and Grants from the Portuguese Science Foundation ( PTDC/BIA-BDE/65625/2006 and PTDC/CVT/69438/2006 ).",

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language = "English",

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AU - Ferreira, Ana Cristina

AU - Almendra, Cláudia

AU - Cardoso, Regina

AU - Pereira, Marta Silva

AU - Beja-Pereira, Albano

AU - Luikart, Gordon

AU - Corrêa de Sá, Maria Inácia

N1 - Funding Information: This work was financed by project Grants from FCT ( PTDC/CVT/69438/2006 and PTDC/CVT/104050/2008 ). CA was supported by a National Grant ( SFRH/BD30737/2006 ) Fundação para a Ciência e Tecnologia (FCT) and GL was partially supported by Grants from the US National Science Foundation (Grant DEB 074218 ), the Walton Family Foundation, CIBIO-UP , and Grants from the Portuguese Science Foundation ( PTDC/BIA-BDE/65625/2006 and PTDC/CVT/69438/2006 ).

PY - 2012/10

Y1 - 2012/10

N2 - A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.

AB - A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.

KW - Bacteriological diagnosis

KW - Brucella suis

KW - Selective medium

KW - Wild boar

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JF - Research in Veterinary Science

IS - 2

ER -

Development and evaluation of a selective medium for Brucella suis

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Keywords

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