TY - JOUR
T1 - Dietary docosahexaenoic acid prevents silica-induced development of pulmonary ectopic germinal centers and glomerulonephritis in the lupus-prone NZBWF1 mouse
AU - Bates, Melissa A.
AU - Akbari, Peyman
AU - Gilley, Kristen N.
AU - Wagner, James G.
AU - Li, Ning
AU - Kopec, Anna K.
AU - Wierenga, Kathryn A.
AU - Jackson-Humbles, Daven
AU - Brandenberger, Christina
AU - Holian, Andrij
AU - Benninghoff, Abby D.
AU - Harkema, Jack R.
AU - Pestka, James J.
N1 - Publisher Copyright:
© 2018 Bates, Akbari, Gilley, Wagner, Li, Kopec, Wierenga, Jackson-Humbles, Brandenberger, Holian, Benninghoff, Harkema and Pestka.
PY - 2018/9/12
Y1 - 2018/9/12
N2 - Ectopic lymphoid structures (ELS) consist of B-cell and T-cell aggregates that are initiated de novo in inflamed tissues outside of secondary lymphoid organs. When organized within follicular dendritic cell (FDC) networks, ELS contain functional germinal centers that can yield autoantibody-secreting plasma cells and promote autoimmune disease. Intranasal instillation of lupus-prone mice with crystalline silica (cSiO 2 ), a respirable particle linked to human lupus, triggers ELS formation in the lung, systemic autoantibodies, and early onset of glomerulonephritis. Here we tested the hypothesis that consumption of docosahexaenoic acid (DHA), an ω-3 polyunsaturated fatty acid with anti-inflammatory properties, influences the temporal profile of cSiO 2 -induced pulmonary ectopic germinal center formation and development of glomerulonephritis. Female NZBWF1 mice (6-wk old) were fed purified isocaloric diets supplemented with 0, 4, or 10 g/kg DHA - calorically equivalent to 0, 2, or 5 g DHA per day consumption by humans, respectively. Beginning at age 8 wk, mice were intranasally instilled with 1 mg cSiO 2 , or saline vehicle alone, once per wk, for 4 wk. Cohorts were sacrificed 1, 5, 9, or 13 wk post-instillation (PI) of the last cSiO 2 dose, and lung and kidney lesions were investigated by histopathology. Tissue fatty acid analyses confirmed uniform dose-dependent DHA incorporation across all cohorts. As early as 1 wk PI, inflammation comprising of B (CD45R + ) and T (CD3 + ) cell accumulation was observed in lungs of cSiO 2 -treated mice compared to vehicle controls; these responses intensified over time. Marked follicular dendritic cell (FDC; CD21 + /CD35 + ) networking appeared at 9 and 13 wk PI. IgG + plasma cells suggestive of mature germinal centers were evident at 13 wk. DHA supplementation dramatically suppressed cSiO 2 -triggered B-cell, T-cell, FDC, and IgG + plasma cell appearance in the lungs as well as anti-dsDNA IgG in bronchial lavage fluid and plasma over the course of the experiment. cSiO 2 induced glomerulonephritis with concomitant B-cell accumulation in the renal cortex at 13 wk PI but this response was abrogated by DHA feeding. Taken together, realistic dietary DHA supplementation prevented initiation and/or progression of ectopic lymphoid neogenesis, germinal center development, systemic autoantibody elevation, and resultant glomerulonephritis in this unique preclinical model of environment-triggered lupus.
AB - Ectopic lymphoid structures (ELS) consist of B-cell and T-cell aggregates that are initiated de novo in inflamed tissues outside of secondary lymphoid organs. When organized within follicular dendritic cell (FDC) networks, ELS contain functional germinal centers that can yield autoantibody-secreting plasma cells and promote autoimmune disease. Intranasal instillation of lupus-prone mice with crystalline silica (cSiO 2 ), a respirable particle linked to human lupus, triggers ELS formation in the lung, systemic autoantibodies, and early onset of glomerulonephritis. Here we tested the hypothesis that consumption of docosahexaenoic acid (DHA), an ω-3 polyunsaturated fatty acid with anti-inflammatory properties, influences the temporal profile of cSiO 2 -induced pulmonary ectopic germinal center formation and development of glomerulonephritis. Female NZBWF1 mice (6-wk old) were fed purified isocaloric diets supplemented with 0, 4, or 10 g/kg DHA - calorically equivalent to 0, 2, or 5 g DHA per day consumption by humans, respectively. Beginning at age 8 wk, mice were intranasally instilled with 1 mg cSiO 2 , or saline vehicle alone, once per wk, for 4 wk. Cohorts were sacrificed 1, 5, 9, or 13 wk post-instillation (PI) of the last cSiO 2 dose, and lung and kidney lesions were investigated by histopathology. Tissue fatty acid analyses confirmed uniform dose-dependent DHA incorporation across all cohorts. As early as 1 wk PI, inflammation comprising of B (CD45R + ) and T (CD3 + ) cell accumulation was observed in lungs of cSiO 2 -treated mice compared to vehicle controls; these responses intensified over time. Marked follicular dendritic cell (FDC; CD21 + /CD35 + ) networking appeared at 9 and 13 wk PI. IgG + plasma cells suggestive of mature germinal centers were evident at 13 wk. DHA supplementation dramatically suppressed cSiO 2 -triggered B-cell, T-cell, FDC, and IgG + plasma cell appearance in the lungs as well as anti-dsDNA IgG in bronchial lavage fluid and plasma over the course of the experiment. cSiO 2 induced glomerulonephritis with concomitant B-cell accumulation in the renal cortex at 13 wk PI but this response was abrogated by DHA feeding. Taken together, realistic dietary DHA supplementation prevented initiation and/or progression of ectopic lymphoid neogenesis, germinal center development, systemic autoantibody elevation, and resultant glomerulonephritis in this unique preclinical model of environment-triggered lupus.
KW - Autoimmunity
KW - Ectopic lymphoid structure
KW - Lung
KW - Omega-3
KW - Polyunsaturated fatty acid
KW - Silica
KW - Systemic lupus erythematosus
UR - http://www.scopus.com/inward/record.url?scp=85053152566&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2018.02002
DO - 10.3389/fimmu.2018.02002
M3 - Article
C2 - 30258439
AN - SCOPUS:85053152566
SN - 1664-3224
VL - 9
JO - Frontiers in Immunology
JF - Frontiers in Immunology
IS - SEP
M1 - 2002
ER -