TY - JOUR
T1 - Disruption of the Borrelia burgdorferi gac gene, encoding the naturally synthesized GyrA C-terminal domain
AU - Knight, Scott W.
AU - Kimmel, Betsy J.
AU - Eggers, Christian H.
AU - Samuels, D. Scott
PY - 2000/4
Y1 - 2000/4
N2 - The C-terminal domain of the A subunit of DNA gyrase, which we term Gac, is naturally synthesized in Borrelia burgdorferi as an abundant DNA-binding protein. Full-length GyrA, which includes the C-terminal domain, is also synthesized by the spirochete and functions as a subunit of DNA gyrase. We have disrupted synthesis of Gac as an independent protein and demonstrated that it is not essential for growth in a coumarin-resistant background. We detected no alterations in DNA maintenance, condensation, or topology in B. burgdorferi lacking this small DNA-binding protein.
AB - The C-terminal domain of the A subunit of DNA gyrase, which we term Gac, is naturally synthesized in Borrelia burgdorferi as an abundant DNA-binding protein. Full-length GyrA, which includes the C-terminal domain, is also synthesized by the spirochete and functions as a subunit of DNA gyrase. We have disrupted synthesis of Gac as an independent protein and demonstrated that it is not essential for growth in a coumarin-resistant background. We detected no alterations in DNA maintenance, condensation, or topology in B. burgdorferi lacking this small DNA-binding protein.
UR - http://www.scopus.com/inward/record.url?scp=0034102967&partnerID=8YFLogxK
U2 - 10.1128/JB.182.7.2048-2051.2000
DO - 10.1128/JB.182.7.2048-2051.2000
M3 - Article
C2 - 10715016
AN - SCOPUS:0034102967
SN - 0021-9193
VL - 182
SP - 2048
EP - 2051
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 7
ER -