Effect of multi-walled carbon nanotube surface modification on bioactivity in the C57BL/6 mouse model

Tina M. Sager; Michael W. Wolfarth; Michael Andrew; Ann Hubbs; Sherri Friend; Teh Hsun Chen; Dale W. Porter; Nianqiang Wu; Feng Yang; Raymond F. Hamilton; Andrij Holian

doi:10.3109/17435390.2013.779757

Effect of multi-walled carbon nanotube surface modification on bioactivity in the C57BL/6 mouse model

Tina M. Sager
, Michael W. Wolfarth
, Michael Andrew
, Ann Hubbs
, Sherri Friend
, Teh Hsun Chen
, Dale W. Porter
, Nianqiang Wu
, Feng Yang
, Raymond F. Hamilton
, Andrij Holian

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

The current study tests the hypothesis that multi-walled carbon nanotubes (MWCNT) with different surface chemistries exhibit different bioactivity profiles in vivo. In addition, the study examined the potential contribution of the NLRP3 inflammasome in MWCNT-induced lung pathology. Unmodified (BMWCNT) and MWCNT that were surface functionalised with -COOH (FMWCNT), were instilled into C57BL/6 mice. The mice were then examined for biomarkers of inflammation and injury, as well as examined histologically for development of pulmonary disease as a function of dose and time. Biomarkers for pulmonary inflammation included cytokines, mediators and the presence of inflammatory cells (IL-1β, IL-18, IL-33, cathepsin B and neutrophils) and markers of injury (albumin and lactate dehydrogenase). The results show that surface modification by the addition of the -COOH group to the MWCNT, significantly reduced the bioactivity and pathogenicity. The results of this study also suggest that in vivo pathogenicity of the BMWCNT and FMWCNT correlates with activation of the NLRP3 inflammasome in the lung.

Original language	English
Pages (from-to)	317-327
Number of pages	11
Journal	Nanotoxicology
Volume	8
Issue number	3
DOIs	https://doi.org/10.3109/17435390.2013.779757
State	Published - May 2014

Funding

The work discussed in this manuscript was supported in part by grant RC2 ES-018742 and NSF grant CBET-0834233. The production and characterisation work was financially supported by a NIH grant (1RC2ES018742-01). The facilities and resources used in this work were partially supported by the NSF grant (EPS 1003907) with matching funds from the West Virginia University Research Corporation and the West Virginia EPSCoR Office. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the National Institute for Occupational Safety and Health.

Funders	Funder number
CBET-0834233, EPS 1003907
RC2ES018742
West Virginia State University

Keywords

Fibrosis
Inflammasome activation
Multi-walled carbon nanotube
Pulmonary toxicity
Surface modification

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10.3109/17435390.2013.779757

Cite this

@article{db4183edc2ee4b2ca69108f0066746fc,

title = "Effect of multi-walled carbon nanotube surface modification on bioactivity in the C57BL/6 mouse model",

abstract = "The current study tests the hypothesis that multi-walled carbon nanotubes (MWCNT) with different surface chemistries exhibit different bioactivity profiles in vivo. In addition, the study examined the potential contribution of the NLRP3 inflammasome in MWCNT-induced lung pathology. Unmodified (BMWCNT) and MWCNT that were surface functionalised with -COOH (FMWCNT), were instilled into C57BL/6 mice. The mice were then examined for biomarkers of inflammation and injury, as well as examined histologically for development of pulmonary disease as a function of dose and time. Biomarkers for pulmonary inflammation included cytokines, mediators and the presence of inflammatory cells (IL-1β, IL-18, IL-33, cathepsin B and neutrophils) and markers of injury (albumin and lactate dehydrogenase). The results show that surface modification by the addition of the -COOH group to the MWCNT, significantly reduced the bioactivity and pathogenicity. The results of this study also suggest that in vivo pathogenicity of the BMWCNT and FMWCNT correlates with activation of the NLRP3 inflammasome in the lung.",

keywords = "Fibrosis, Inflammasome activation, Multi-walled carbon nanotube, Pulmonary toxicity, Surface modification",

author = "Sager, \{Tina M.\} and Wolfarth, \{Michael W.\} and Michael Andrew and Ann Hubbs and Sherri Friend and Chen, \{Teh Hsun\} and Porter, \{Dale W.\} and Nianqiang Wu and Feng Yang and Hamilton, \{Raymond F.\} and Andrij Holian",

note = "Funding Information: The work discussed in this manuscript was supported in part by grant RC2 ES-018742 and NSF grant CBET-0834233. The production and characterisation work was financially supported by a NIH grant (1RC2ES018742-01). The facilities and resources used in this work were partially supported by the Funding Information: NSF grant (EPS 1003907) with matching funds from the West Virginia University Research Corporation and the West Virginia EPSCoR Office. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the National Institute for Occupational Safety and Health.",

year = "2014",

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language = "English",

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AU - Wolfarth, Michael W.

AU - Andrew, Michael

AU - Hubbs, Ann

AU - Friend, Sherri

AU - Chen, Teh Hsun

AU - Porter, Dale W.

AU - Wu, Nianqiang

AU - Yang, Feng

AU - Hamilton, Raymond F.

AU - Holian, Andrij

N1 - Funding Information: The work discussed in this manuscript was supported in part by grant RC2 ES-018742 and NSF grant CBET-0834233. The production and characterisation work was financially supported by a NIH grant (1RC2ES018742-01). The facilities and resources used in this work were partially supported by the Funding Information: NSF grant (EPS 1003907) with matching funds from the West Virginia University Research Corporation and the West Virginia EPSCoR Office. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the National Institute for Occupational Safety and Health.

PY - 2014/5

Y1 - 2014/5

N2 - The current study tests the hypothesis that multi-walled carbon nanotubes (MWCNT) with different surface chemistries exhibit different bioactivity profiles in vivo. In addition, the study examined the potential contribution of the NLRP3 inflammasome in MWCNT-induced lung pathology. Unmodified (BMWCNT) and MWCNT that were surface functionalised with -COOH (FMWCNT), were instilled into C57BL/6 mice. The mice were then examined for biomarkers of inflammation and injury, as well as examined histologically for development of pulmonary disease as a function of dose and time. Biomarkers for pulmonary inflammation included cytokines, mediators and the presence of inflammatory cells (IL-1β, IL-18, IL-33, cathepsin B and neutrophils) and markers of injury (albumin and lactate dehydrogenase). The results show that surface modification by the addition of the -COOH group to the MWCNT, significantly reduced the bioactivity and pathogenicity. The results of this study also suggest that in vivo pathogenicity of the BMWCNT and FMWCNT correlates with activation of the NLRP3 inflammasome in the lung.

AB - The current study tests the hypothesis that multi-walled carbon nanotubes (MWCNT) with different surface chemistries exhibit different bioactivity profiles in vivo. In addition, the study examined the potential contribution of the NLRP3 inflammasome in MWCNT-induced lung pathology. Unmodified (BMWCNT) and MWCNT that were surface functionalised with -COOH (FMWCNT), were instilled into C57BL/6 mice. The mice were then examined for biomarkers of inflammation and injury, as well as examined histologically for development of pulmonary disease as a function of dose and time. Biomarkers for pulmonary inflammation included cytokines, mediators and the presence of inflammatory cells (IL-1β, IL-18, IL-33, cathepsin B and neutrophils) and markers of injury (albumin and lactate dehydrogenase). The results show that surface modification by the addition of the -COOH group to the MWCNT, significantly reduced the bioactivity and pathogenicity. The results of this study also suggest that in vivo pathogenicity of the BMWCNT and FMWCNT correlates with activation of the NLRP3 inflammasome in the lung.

KW - Fibrosis

KW - Inflammasome activation

KW - Multi-walled carbon nanotube

KW - Pulmonary toxicity

KW - Surface modification

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DO - 10.3109/17435390.2013.779757

M3 - Article

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SN - 1743-5390

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JO - Nanotoxicology

JF - Nanotoxicology

IS - 3

ER -

Effect of multi-walled carbon nanotube surface modification on bioactivity in the C57BL/6 mouse model

Abstract

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Keywords

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