Effect of pendant chain lengths and backbone functionalities on the chemical selectivity of sulfonated amphiphilic copolymers as pseudo-stationary phases in electrokinetic chromatography

Wei Shi, Dominic S. Peterson, Christopher P. Palmer

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Amphiphilic copolymers of AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid) and hydrophobic monomers with various chemical structures were synthesized, characterized and used as novel electrokinetic chromatography polymeric pseudo-stationary phases, showing significant chemical selectivity differences from that of the conventional monomeric pseudo-stationary phase, sodium lauryl sulphate. Copolymers of AMPS and methacrylates with different pendant chain lengths (C8, C12 and C18) were investigated and no significant difference in chemical selectivity was observed among them. However, the spacer bonding chemistry was shown to contribute to significant chemical selectivity difference, e.g. poly(AMPS-lauryl methacrylate) showed different chemical selectivity from poly(AMPS-lauryl methacrylamide). Linear solvation energy relationship analysis of 20 solutes by eight different polymeric pseudo-stationary phases was employed to investigate the solute molecule structural contributions to the retention. Hydrogen-bonding properties (described by system constants b and a) of poly(AMPS-alkyl methacrylamide) were found stronger than those of poly(AMPS-alkyl methacrylate).

Original languageEnglish
Pages (from-to)123-135
Number of pages13
JournalJournal of Chromatography A
Volume924
Issue number1-2
DOIs
StatePublished - Jul 27 2001

Keywords

  • Electrokinetic chromatography
  • Linear solvation energy relationships
  • Methacrylamide
  • Methacrylate
  • Pseudo-stationary phases

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