TY - JOUR
T1 - Effect of pendent group structures on the chemical selectivity and performance of sulfonated copolymers as novel pseudophases in electrokinetic chromatography
AU - Shi, Wei
AU - Palmer, Christopher P.
PY - 2002
Y1 - 2002
N2 - Amphiphilic copolymers of 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS) with dihydrocholesteryl acrylate (pDHCHAt) or tert-octyl acrylamide (ptOAm) as comonomers were synthesized, characterized and used as new polymeric pseudostationary phases in electrokinetic chromatography. High-efficiency separations with theoretical plates around 500 000 to 870 000/m were achieved. Linear solvation energy relationships analysis of 20 solutes was performed to investigate the retention mechanism. Polycyclic aromatic hydrocarbons (PAHs) were separated under 30% v/v acetonitrile. The separation of nine aromatic model solutes by pDHCHAt showed significantly different selectivity from that of sodium dodecyl sulfate. Although only minor difference in selectivity was found for small aromatic compounds on pDHCHAt and poly(AMPS-lauryl acrylate), significantly different retention mechanism was found for PAHs and n-dodecanophenone comparing both copolymers. Significant chemical selectivity difference was found for selected small aromatic compounds on ptOAm and poly(AMPS-stearyl acrylamide).
AB - Amphiphilic copolymers of 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS) with dihydrocholesteryl acrylate (pDHCHAt) or tert-octyl acrylamide (ptOAm) as comonomers were synthesized, characterized and used as new polymeric pseudostationary phases in electrokinetic chromatography. High-efficiency separations with theoretical plates around 500 000 to 870 000/m were achieved. Linear solvation energy relationships analysis of 20 solutes was performed to investigate the retention mechanism. Polycyclic aromatic hydrocarbons (PAHs) were separated under 30% v/v acetonitrile. The separation of nine aromatic model solutes by pDHCHAt showed significantly different selectivity from that of sodium dodecyl sulfate. Although only minor difference in selectivity was found for small aromatic compounds on pDHCHAt and poly(AMPS-lauryl acrylate), significantly different retention mechanism was found for PAHs and n-dodecanophenone comparing both copolymers. Significant chemical selectivity difference was found for selected small aromatic compounds on ptOAm and poly(AMPS-stearyl acrylamide).
KW - Dihydrocholesteryl acrylate
KW - Electrokinetic chromatography
KW - Linear solvation energy relationshi
KW - Polycyclic aromatic hydrocarbons
KW - Tert-Octyl acrylamide
UR - http://www.scopus.com/inward/record.url?scp=0036261460&partnerID=8YFLogxK
U2 - 10.1002/1522-2683(200205)23:9<1285::AID-ELPS1285>3.0.CO;2-A
DO - 10.1002/1522-2683(200205)23:9<1285::AID-ELPS1285>3.0.CO;2-A
M3 - Article
C2 - 12007128
AN - SCOPUS:0036261460
SN - 0173-0835
VL - 23
SP - 1285
EP - 1295
JO - Electrophoresis
JF - Electrophoresis
IS - 9
ER -