Effect of resistance exercise and carbohydrate ingestion on oxidative stress

Steven R. McAnulty, Lisa S. McAnulty, David C. Nieman, Jason D. Morrow, Alan C. Utter, Charles L. Dumke

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Some research studies have produced data indicating that resistance exercise induces oxidative stress, despite minimal increases in VO2. These studies have primarily relied on oxidative stress markers with low sensitivity and debatable reliability. However, F2-isoprostanes as measured by gas chromatography mass spectrometry are considered to be a reliable and precise indicator of oxidative stress. Carbohydrate ingestion during exercise is associated with reduced levels of stress hormones, which may influence oxidative stress and plasma antioxidant potential. Therefore, the purpose of this study was to investigate the influence of carbohydrate ingestion during resistance training on F2-isoprostanes and plasma antioxidant potential. Thirty strength-trained subjects were randomized to carbohydrate (CHO) or placebo (PLA) groups that lifted weights for 2 h. Subjects received 10 ml kg-1 h-1 CHO (6%) or PLA beverages during the exercise. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and analyzed for cortisol as a marker of general stress, F2-isoprostanes as a measure of oxidative stress, and ferric reducing ability of plasma (FRAP) as a measure of antioxidant potential, and for muscle glycogen, respectively. Decreases in muscle glycogen content did not differ between CHO and PLA. Cortisol and FRAP increased significantly in CHO and PLA (P = 0.008 and 0.044, respectively), but the pattern of change was not different between groups. F2-isoprostanes were unaffected by exercise. These results indicate that exhaustive resistance exercise and carbohydrate ingestion have no effect on oxidative stress or plasma antioxidant potential in trained subjects.

Original languageEnglish
Pages (from-to)1219-1224
Number of pages6
JournalFree Radical Research
Issue number11
StatePublished - Nov 2005


  • Antioxidant potential
  • Cortisol
  • F-isoprostanes
  • Muscle glycogen


Dive into the research topics of 'Effect of resistance exercise and carbohydrate ingestion on oxidative stress'. Together they form a unique fingerprint.

Cite this