TY - JOUR
T1 - Effects of repeated bouts of long-duration endurance exercise on muscle and urinary levels of 8-hydroxy-2ʹ-deoxyguanosine in moderately trained cyclists
AU - Yasuda, Nobuo
AU - Bolin, Celeste
AU - Cardozo-Pelaez, Fernando
AU - Ruby, Brent C.
N1 - Publisher Copyright:
© 2015 Taylor & Francis.
PY - 2015/10/2
Y1 - 2015/10/2
N2 - Abstract: The purpose of this study was to determine the effects of repeated bouts of long-duration endurance exercise on both muscle and urinary levels of oxidative DNA damage in moderately trained individuals. Seven moderately trained male cyclists participated in this study. All participants repeated two sessions consisting of a 5-h cycling period (equivalent to approximately 52% (Formula presented.) O2peak) followed by a 15-h rest, then a 40-km time trial. During the sessions, participants were instructed to take water ad libitum and to consume a standard sports drink consisting of 0.12 g·kg−1 body weight·hr−1 of carbohydrates. For each session, 24 h urine output was collected on the day before the 5-h exercise, and also between the 5-h exercise and 40-km time trial, in addition to between days 1–5 post-exercise. Subsequently, muscle and urinary levels of 8-hydroxy-2ʹ- deoxyguanosine (8-OHdG) were determined using high performance liquid chromatography with electrochemical detection. No significant alterations were observed between two sessions at the muscle or urinary levels of 8-OHdG. These results suggest that repeated bouts of exercise with a 7-day washout period may not lead to an accumulation of DNA damage products after a second 5-h stationary cycling bout.
AB - Abstract: The purpose of this study was to determine the effects of repeated bouts of long-duration endurance exercise on both muscle and urinary levels of oxidative DNA damage in moderately trained individuals. Seven moderately trained male cyclists participated in this study. All participants repeated two sessions consisting of a 5-h cycling period (equivalent to approximately 52% (Formula presented.) O2peak) followed by a 15-h rest, then a 40-km time trial. During the sessions, participants were instructed to take water ad libitum and to consume a standard sports drink consisting of 0.12 g·kg−1 body weight·hr−1 of carbohydrates. For each session, 24 h urine output was collected on the day before the 5-h exercise, and also between the 5-h exercise and 40-km time trial, in addition to between days 1–5 post-exercise. Subsequently, muscle and urinary levels of 8-hydroxy-2ʹ- deoxyguanosine (8-OHdG) were determined using high performance liquid chromatography with electrochemical detection. No significant alterations were observed between two sessions at the muscle or urinary levels of 8-OHdG. These results suggest that repeated bouts of exercise with a 7-day washout period may not lead to an accumulation of DNA damage products after a second 5-h stationary cycling bout.
KW - DNA oxidation
KW - apoptosis
KW - exercise intensity
KW - oxidative stress
KW - ultra-endurance exercise
UR - http://www.scopus.com/inward/record.url?scp=84931578558&partnerID=8YFLogxK
U2 - 10.1080/02640414.2015.1004637
DO - 10.1080/02640414.2015.1004637
M3 - Article
C2 - 25620316
AN - SCOPUS:84931578558
SN - 0264-0414
VL - 33
SP - 1692
EP - 1701
JO - Journal of Sports Sciences
JF - Journal of Sports Sciences
IS - 16
ER -