Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

Taylor M. Wilcox; Kevin S. McKelvey; Michael K. Young; Winsor H. Lowe; Michael K. Schwartz

doi:10.1007/s12686-015-0465-z

Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

Taylor M. Wilcox, Kevin S. McKelvey, Michael K. Young, Winsor H. Lowe, Michael K. Schwartz

Biological Sciences

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.

Original language	English
Pages (from-to)	639-641
Number of pages	3
Journal	Conservation Genetics Resources
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/s12686-015-0465-z
State	Published - Sep 18 2015

Keywords

Detection
Fish
Mitochondria
eDNA

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10.1007/s12686-015-0465-z

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title = "Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)",

abstract = "Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.",

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T1 - Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

AU - Wilcox, Taylor M.

AU - McKelvey, Kevin S.

AU - Young, Michael K.

AU - Lowe, Winsor H.

AU - Schwartz, Michael K.

PY - 2015/9/18

Y1 - 2015/9/18

N2 - Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.

AB - Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.

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KW - Fish

KW - Mitochondria

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Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

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Keywords

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