Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

Taylor M. Wilcox, Kevin S. McKelvey, Michael K. Young, Winsor H. Lowe, Michael K. Schwartz

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.

Original languageEnglish
Pages (from-to)639-641
Number of pages3
JournalConservation Genetics Resources
Volume7
Issue number3
DOIs
StatePublished - Sep 18 2015

Keywords

  • Detection
  • Fish
  • Mitochondria
  • eDNA

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