Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

Taylor M. Wilcox; Kevin S. McKelvey; Michael K. Young; Winsor H. Lowe; Michael K. Schwartz

doi:10.1007/s12686-015-0465-z

Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

Taylor M. Wilcox
, Kevin S. McKelvey
, Michael K. Young
, Winsor H. Lowe
, Michael K. Schwartz

Division of Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.

Original language	English
Pages (from-to)	639-641
Number of pages	3
Journal	Conservation Genetics Resources
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/s12686-015-0465-z
State	Published - Sep 18 2015

Funding

TW is supported by a NSF Graduate Research Fellowship (Grant No. DGE-1313190). This work was done in accordance with Montana Fish, Wildlife and Parks Scientific Collectors Permit #SCP-33-14. This manuscript was improved by comments from Kellie Carim, Katie Zarn, and two anonymous reviewers.

Funder number
DGE-1313190

Keywords

Detection
Fish
Mitochondria
eDNA

Access to Document

10.1007/s12686-015-0465-z

Cite this

@article{fb8020bbc57c4cabbd8b2db378a8dc0d,

title = "Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)",

abstract = "Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.",

keywords = "Detection, Fish, Mitochondria, eDNA",

author = "Wilcox, \{Taylor M.\} and McKelvey, \{Kevin S.\} and Young, \{Michael K.\} and Lowe, \{Winsor H.\} and Schwartz, \{Michael K.\}",

note = "Publisher Copyright: {\textcopyright} 2015, Springer Science+Business Media Dordrecht.",

year = "2015",

month = sep,

day = "18",

doi = "10.1007/s12686-015-0465-z",

language = "English",

volume = "7",

pages = "639--641",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer Science and Business Media B.V.",

number = "3",

}

TY - JOUR

T1 - Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

AU - Wilcox, Taylor M.

AU - McKelvey, Kevin S.

AU - Young, Michael K.

AU - Lowe, Winsor H.

AU - Schwartz, Michael K.

PY - 2015/9/18

Y1 - 2015/9/18

N2 - Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.

AB - Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2–10-μm particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.

KW - Detection

KW - Fish

KW - Mitochondria

KW - eDNA

UR - https://www.scopus.com/pages/publications/84939148619

U2 - 10.1007/s12686-015-0465-z

DO - 10.1007/s12686-015-0465-z

M3 - Article

AN - SCOPUS:84939148619

SN - 1877-7252

VL - 7

SP - 639

EP - 641

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 3

ER -

Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this