Exercise-and cold-induced human pgc-1α mrna isoform specific responses

Camille Larson; Megan Opichka; Mark L. McGlynn; Christopher W. Collins; Dustin Slivka

doi:10.3390/ijerph17165740

Exercise-and cold-induced human pgc-1α mrna isoform specific responses

Camille Larson
, Megan Opichka
, Mark L. McGlynn
, Christopher W. Collins
, Dustin Slivka

School of Integrative Physiology and Athletic Training

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise-and temperature-induced PGC-1α response.

Original language	English
Article number	5740
Pages (from-to)	1-10
Number of pages	10
Journal	International Journal of Environmental Research and Public Health
Volume	17
Issue number	16
DOIs	https://doi.org/10.3390/ijerph17165740
State	Published - Aug 2 2020

Funding

Funding: This publication was made possible by grants from the National Institute for General Medical Science (NIGMS; P20GM103427), a component of the National Institutes of Health (NIH), and its contents are the sole responsibility of the authors and do not necessarily represent the official views of NIGMS or NIH. Additional funding for this project was provided by the University of Nebraska at Omaha’s Office of Research and Creative Activity Fund for Undergraduate Scholarly Experiences (FUSE) and the NASA-Nebraska Space Grant and its contents are the sole responsibility of the authors.

Funders	Funder number
University of Nebraska-Lincoln
P20GM103427

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Alternate promoter
Primary promoter
RT-qPCR
Transcription
Truncated

Access to Document

10.3390/ijerph17165740

Cite this

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title = "Exercise-and cold-induced human pgc-1α mrna isoform specific responses",

abstract = "Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65\% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise-and temperature-induced PGC-1α response.",

keywords = "Alternate promoter, Primary promoter, RT-qPCR, Transcription, Truncated",

author = "Camille Larson and Megan Opichka and McGlynn, \{Mark L.\} and Collins, \{Christopher W.\} and Dustin Slivka",

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AU - Slivka, Dustin

PY - 2020/8/2

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AB - Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise-and temperature-induced PGC-1α response.

KW - Alternate promoter

KW - Primary promoter

KW - RT-qPCR

KW - Transcription

KW - Truncated

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ER -

Exercise-and cold-induced human pgc-1α mrna isoform specific responses

Abstract

Funding

UN SDGs

Keywords

Access to Document

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