Exercise does not increase cyclooxygenase-2 myocardial levels in young or senescent hearts

John C. Quindry, Joel French, Karyn L. Hamilton, Youngil Lee, Josh Selsby, Scott Powers

Research output: Contribution to journalArticlepeer-review

Abstract

Increased myocardial cyclooxygenase-2 (COX-2)activity is essential for late phase ischemic preconditioning (IPC). Currently unknowniswhether cardioprotection elicited by exercise also involves elevated myocardial COX-2 activity. This investigationtested whether aerobic exercise elevates myocardial COX-2 protein content or enzyme activity in young and senescent male Fisher 344 rats assigned to sedentary or cardioprotective endurance exercise treatments (3 consecutive days of treadmill exercise, 60 min/day ∼70% VO2max). Assay of cardiac COX-2 protein content, catalytic activity, and inducible nitric oxide synthase (iNOS) protein content reveal that exercise did not alter COX-2 activity (PGE2, p = 0.866; PGF1α, p = 0.796) or protein levels (p = 0.397) within young or senescent hearts. In contrast, myocardial iNOS, an up-stream mediator of COX-2 expression, was over-expressed by an average of 37% in aged hearts (p = 0.005), though iNOS was not influenced by exercise. Findings reveal exercise does not elevate cardiac COX-2 activity and suggests that mechanisms responsible for cardioprotection differ between IPC and aerobic exercise.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalJournal of Physiological Sciences
Volume60
Issue number3
DOIs
StatePublished - May 2010

Keywords

  • Aging
  • Exercise
  • Myocardial
  • Physical activity
  • Prostaglandin
  • Senescence

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