Experimental woodsmoke exposure during exercise and blood oxidative stress

Bridget Peters, Christopher Ballmann, Tiffany Quindry, Emily G. Zehner, Justin McCroskey, Matthew Ferguson, Tony Ward, Charles Dumke, John C. Quindry

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Objectives: The current laboratory study quantified blood oxidative stress to woodsmoke exposure. Methods: Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 mg/m3], Low Exposure [250 mg/m3], and High Exposure [500 mg/m3], Woodsmoke [particulate matter less than 2.5 mm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples. Results: UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air. Conclusion: Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.

Original languageEnglish
Pages (from-to)1073-1081
Number of pages9
JournalJournal of Occupational and Environmental Medicine
Issue number12
StatePublished - 2018


  • Air pollution
  • Antioxidants
  • Free radicals
  • Reactive oxygen species


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