TY - JOUR
T1 - Experimental woodsmoke exposure during exercise and blood oxidative stress
AU - Peters, Bridget
AU - Ballmann, Christopher
AU - Quindry, Tiffany
AU - Zehner, Emily G.
AU - McCroskey, Justin
AU - Ferguson, Matthew
AU - Ward, Tony
AU - Dumke, Charles
AU - Quindry, John C.
N1 - Publisher Copyright:
Copyright © 2018 American College of Occupational and Environmental Medicine
PY - 2018
Y1 - 2018
N2 - Objectives: The current laboratory study quantified blood oxidative stress to woodsmoke exposure. Methods: Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 mg/m3], Low Exposure [250 mg/m3], and High Exposure [500 mg/m3], Woodsmoke [particulate matter less than 2.5 mm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples. Results: UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air. Conclusion: Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.
AB - Objectives: The current laboratory study quantified blood oxidative stress to woodsmoke exposure. Methods: Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 mg/m3], Low Exposure [250 mg/m3], and High Exposure [500 mg/m3], Woodsmoke [particulate matter less than 2.5 mm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples. Results: UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air. Conclusion: Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.
KW - Air pollution
KW - Antioxidants
KW - Free radicals
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85058547739&partnerID=8YFLogxK
U2 - 10.1097/JOM.0000000000001437
DO - 10.1097/JOM.0000000000001437
M3 - Article
C2 - 30188494
AN - SCOPUS:85058547739
SN - 1076-2752
VL - 60
SP - 1073
EP - 1081
JO - Journal of Occupational and Environmental Medicine
JF - Journal of Occupational and Environmental Medicine
IS - 12
ER -
