Genetic probes of ribosomal RNA function.

M. O'Connor; C. A. Brunelli; M. A. Firpo; S. T. Gregory; K. R. Lieberman; J. S. Lodmell; H. Moine; D. I. Van Ryk; A. E. Dahlberg

doi:10.1139/o95-093

Genetic probes of ribosomal RNA function.

M. O'Connor, C. A. Brunelli, M. A. Firpo, S. T. Gregory, K. R. Lieberman, J. S. Lodmell, H. Moine, D. I. Van Ryk, A. E. Dahlberg

Brown University

Research output: Contribution to journal › Review article › peer-review

51 Scopus citations

Abstract

We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.

Original language	English
Pages (from-to)	859-868
Number of pages	10
Journal	Biochemistry and Cell Biology
Volume	73
Issue number	11-12
DOIs	https://doi.org/10.1139/o95-093
State	Published - 1995

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title = "Genetic probes of ribosomal RNA function.",

abstract = "We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.",

author = "M. O'Connor and Brunelli, {C. A.} and Firpo, {M. A.} and Gregory, {S. T.} and Lieberman, {K. R.} and Lodmell, {J. S.} and H. Moine and {Van Ryk}, {D. I.} and Dahlberg, {A. E.}",

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AU - O'Connor, M.

AU - Brunelli, C. A.

AU - Firpo, M. A.

AU - Gregory, S. T.

AU - Lieberman, K. R.

AU - Lodmell, J. S.

AU - Moine, H.

AU - Van Ryk, D. I.

AU - Dahlberg, A. E.

PY - 1995

Y1 - 1995

N2 - We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.

AB - We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.

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DO - 10.1139/o95-093

M3 - Review article

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VL - 73

SP - 859

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JO - Biochemistry and Cell Biology

JF - Biochemistry and Cell Biology

IS - 11-12

ER -

Genetic probes of ribosomal RNA function.

Abstract

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