TY - JOUR
T1 - groEL expression in gyrB mutants of Borrelia burgdorferi
AU - Alverson, Janet
AU - Samuels, D. Scott
PY - 2002/11
Y1 - 2002/11
N2 - GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi. Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.
AB - GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi. Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.
UR - https://www.scopus.com/pages/publications/0036838143
U2 - 10.1128/JB.183.21.6069-6072.2002
DO - 10.1128/JB.183.21.6069-6072.2002
M3 - Article
C2 - 12374843
AN - SCOPUS:0036838143
SN - 0021-9193
VL - 184
SP - 6069
EP - 6072
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 21
ER -